• Login
    ARAN - Access to Research at NUI Galway
    View Item 
    •   ARAN Home
    • Support Services
    • Externally hosted open access publications with NUI Galway authors (2)
    • View Item
    •   ARAN Home
    • Support Services
    • Externally hosted open access publications with NUI Galway authors (2)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ARANCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsTypesThis CollectionBy Issue DateAuthorsTitlesSubjectsTypes

    My Account

    LoginRegister

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Help

    How to submit and FAQs

    Ageing octopods from stylets: development of a technique for permanent preparations

    Thumbnail
    View/Open
    Full Text
    Date
    2010-05-25
    Author
    Barratt, I. M.
    Allcock, A. L.
    Metadata
    Show full item record
    Usage
    This item's downloads: 0 (view details)
    Cited 23 times in Scopus (view citations)
    
    Recommended Citation
    Barratt, I. M. Allcock, A. L. (2010). Ageing octopods from stylets: development of a technique for permanent preparations. ICES Journal of Marine Science 67 (7), 1452-1457
    Published Version
    https://academic.oup.com/icesjms/article-pdf/67/7/1452/1905074/fsq047.pdf
    Abstract
    Previous attempts at ageing octopods from stylets have relied on preparations that deteriorate with time. Some techniques require an immediate photographic record, others allow real-time enumeration but do not provide a permanent archive. A technique is described that produces permanent and archivable preparations of octopod stylets. Stylets were dehydrated in ethanol and infiltrated with a low-viscosity resin. Subsequent polymerization of the resin allowed the embedded stylet to be ground and polished to reveal the stylet microstructure. This comprised increments that are probably suitable for age estimation. The technique was developed using stylets of Octopus vulgaris and Eledone cirrhosa. Increments were composed of light and dark bands and were clearly defined at x400 and at x625 magnifications. The number of increments ranged from 189 to 399. The stylets of a deep-sea species (Bathypolypus sponsalis) and an Antarctic species (Megaleledone setebos) were also examined. Each appeared to have growth increments, despite the perception that the environments they inhabited may not provide daily cues. Using the technique developed, the pre-hatch nucleus was seldom well defined, as reported for O. pallidus, stylets of which were prepared using a non-permanent method. Reasons for this are discussed. The microstructure clarity revealed is probably associated with the ultra-low viscosity of the resin used.
    URI
    http://hdl.handle.net/10379/10359
    Collections
    • Externally hosted open access publications with NUI Galway authors (2)
    • Copyright @ NUI Galway
    • Library
    • NUI Galway