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dc.contributor.authorAiyaz, Mohammed
dc.contributor.authorThimmappa Divakara, Shetty
dc.contributor.authorMudili, Venkataramana
dc.contributor.authorGeorge Moore, Geromy
dc.contributor.authorKumar Gupta, Vijai
dc.contributor.authori Yli-Mattila, Tapan
dc.contributor.authorChandra Nayaka, Siddaiah
dc.contributor.authorRamachandrappa Niranjana, Siddapura
dc.identifier.citationAiyaz, Mohammed; Thimmappa Divakara, Shetty; Mudili, Venkataramana; George Moore, Geromy; Kumar Gupta, Vijai; i Yli-Mattila, Tapan; Chandra Nayaka, Siddaiah; Ramachandrappa Niranjana, Siddapura (2016). Molecular diversity of seed-borne fusarium species associated with maize in india. Current Genomics 17 (2), 132-144
dc.description.abstractA total of 106 maize seed samples were collected from different agro-climatic regions of India. Sixty-two Fusarium isolates were recovered, 90% of which were identified as Fusarium verticillioides based on morphological and molecular characters. Use of the tef-1 alpha gene corrected/refined the morphological species identifications of 11 isolates, and confirmed those of the remaining isolates. Genetic diversity among the Fusarium isolates involved multilocus fingerprinting profiles by Inter Simple Sequence Repeats (ISSR) UP-GMA and tef-1 alpha gene phenetic analyses; for which, we observed no significant differences among the isolates based on geographic origin or fumonisin production; most of the subdivision related to species. Genotyping was performed on the F. verticillioides isolates, using 12 primer sets from the fumonisin pathway, to elucidate the molecular basis of fumonisin production or non-production. One fumonisin-negative isolate, UOMMF-16, was unable to amplify nine of the 12 fumonisin cluster genes tested. We also used the CD-ELISA method to confirm fumonisin production for our 62 Fusarium isolates. Only 15 isolates were found to be fumonisin-negative. Interestingly, genotypic characterization revealed six isolates with various gene deletion patterns that also tested positive for the production of fumonisins via CD-ELISA. Our findings confirm the importance of molecular studies for species delimitation, and for observing genetic and phenotypic diversity, among the Fusaria.
dc.publisherBentham Science Publishers Ltd.
dc.relation.ispartofCurrent Genomics
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.subjecttef-1 alpha gene
dc.subjectinter simple sequence repeats
dc.subjectfumonisin gene cluster
dc.subjectpolyketide synthase gene
dc.subjectneural-tube defects
dc.subjectfumonisin production
dc.subjectmycotoxin contamination
dc.subjectsequence alignment
dc.subjectsouthern india
dc.titleMolecular diversity of seed-borne fusarium species associated with maize in india

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