National Centre for Biomedical Engineering Science (Scholarly Articles)
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Differential release of high mannose structural isoforms by fungal and bacterial endo-β-N-acetylglucosaminidases
(Royal Society of Chemistry, 2012-02-28)Endo-beta-N-acetylglucosaminidases (ENGases) are widely used to remove N-linked oligosaccharides from glycoproteins for glycomic and proteomic studies and biopharmaceutical processes. Although several ENGases are widely ... -
Campylobacter jejuni strain discrimination and temperature-dependent glycome expression profiling by lectin microarray
(Elsevier, 2014-02-12)Gram-negative Campylobacter jejuni is the leading cause of bacterial gastroenteritis in humans worldwide and the most frequently identified infectious trigger in patients developing Guillain-Barré syndrome (GBS). While ... -
Complex and dynamic transcriptional changes allow the helminth Fasciola gigantica to adjust to its intermediate snail and definitive mammalian hosts
(BMC (part of Springer Nature), 2019-10-12)The tropical liver fluke, Fasciola gigantica causes fasciolosis, an important disease of humans and livestock. We characterized dynamic transcriptional changes associated with the development of the parasite in its two ... -
Effects of combined progesterone and 17 beta-estradiol treatment on the transcriptome of cultured human myometrial smooth muscle cells
(American Physiological Society, 2016-01-01)A transcriptomic analysis of cultured human uterine smooth muscle cells (hUtSMCs) was performed to examine gene expression profiles in smooth muscle in an environment containing the two major steroid hormones that regulate ... -
Development of a rapid recombinase polymerase amplification assay for the detection of Streptococcus pneumoniae in whole blood
(BioMed Central, 2015-10-29)Background: Streptococcus pneumoniae is an important cause of microbial disease in humans. The introduction of multivalent vaccines has coincided with a dramatic decrease in the number of pneumococcal-related deaths. In ... -
Alignment-free clustering of transcription factor binding motifs using a genetic-k-medoids approach
(BioMed Central, 2015-01-28)Background: Familial binding profiles (FBPs) represent the average binding specificity for a group of structurally related DNA-binding proteins. The construction of such profiles allows the classification of novel motifs ... -
Analysis of stress-induced hepatic gene expression in rainbow trout (Oncorhynchus mykiss) selected for high- and low-responsiveness to stress
(Elsevier, 2011-12)The production and welfare of intensively reared fish would be improved by reducing stress responsiveness. One approach to achieving this goal is selective breeding utilising stress-responsive genes as direct genetic markers ... -
A note on the evaluation of a beta-casein variant in bovine breeds by allele-specific PCR and relevance to beta-casomorphin
(Teagasc; De Gruyter Open, 2008)Two genetic variants of the bovine beta-casein gene (A(1) and B) encode a histidine residue at codon 67, resulting in potential liberation of a bioactive peptide, beta-casomorphin, upon digestion. An allele-specific PCR ... -
A single nucleotide polymorphism in the bovine beta-casein promoter region across different bovine breeds
(Cambridge Journals, 2006-02-14)The bovine β-casein (CSN2) gene has been shown to span a region of 8·5 kb, containing nine exons and eight intervening introns (Bonsing et al. 1988; Martin et al. 2002). The exons range in size from 24 to 498 bp; the ... -
Isolation and characterization of the bovine Stearoyl-CoAdesaturase promoter and analysis of polymorphisms in the promoter region in dairy cows
(Springer, 2005-03)Conjugated linoleic acid (CLA) in milk arises through microbial biohydrogenation of dietary polyunsaturated fatty acids (PUFA) in the rumen, and by the action of mammary Stearoyl-CoA desaturase (Scd). A large variation ... -
Bovine kappa-casein gene promoter haplotypes with potential implications for milk protein expression
(Elsevier ScienceDirect, 2007-05-25)Genetic analysis of the kappa-casein gene (CSN3) promoter regions of 42 cattle representing 9 different breeds revealed that 2 distinct haplotypes (A and B) exist at this locus, differing from each other by single base ... -
Cloning and characterization of two genes encoding rainbow trout homologues of the IFITM protein family
(Elsevier, 2006-04-15)To identify genes connected with leucocyte function in rainbow trout a cDNA library derived front head kidney leucocytes exposed to phytohemagglutinin (PHA) was screened. A clone with homology to the previously described ... -
A cDNA microarray assessment of gene expression in the liver of rainbow trout (Oncorhynchus mykiss) in response to a handling and confinement stressor
(Elsevier, 2008-03)A purpose-designed microarray platform (Stressgenes, Phase 1) was utilised to investigate the changes in gene expression within the liver of rainbow trout during exposure to a prolonged period of confinement. Tissue and ... -
Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (Sparus aurata L.)
(BioMed Central, 2010-03-22)Background: Selection programs for growth and stress traits in cultured fish are fundamental to the improvement of aquaculture production. The gilthead sea bream ( Sparus aurata) is the main aquacultured species in the ... -
Effects of combined progesterone and beta-estradiol treatment on the transcriptome of cultured human myometrial smooth muscle cells
(American Physiological Society, 2015)A transcriptomic analysis of cultured human uterine smooth muscle cells (hUtSMCs) was performed in order to examine gene expression profiles in smooth muscle in an environment containing the two major steroid hormones that ... -
Dietary vegetable oils do not alter the intestine transcriptome of gilthead sea bream (Sparus aurata), but modulate the transcriptomic response to infection with Enteromyxum leei
(BioMed Central, 2012)Background: Studies conducted with gilthead sea bream (Sparus aurata L.) have determined the maximum dietary replacement of fish meal and oil without compromising growth or product quality. The present study aimed to analyze ... -
Interaction of cell culture with composition effects on the mechanical properties of polycaprolactone-hydroxyapatite scaffolds fabricated via selective laser sintering (SLS)
(Elsevier, 2012)In the current study PCL/HA composites were fabricated using SLS as two- and three-dimensional lattice structures and exposed to a cellular component (MC 313 osteoblast-like cells). The main aims were to determine the ... -
Finite element predictions compared to experimental results for the effective modulus of bone tissue engineering scaffolds fabricated by selective laser sintering
(Springer Verlag, 2009)A current challenge in bone tissue engineering is to create scaffolds with suitable mechanical properties, high porosity, full interconnectivity and suitable pore size. In this paper, polyamide and polycaprolactone scaffolds ... -
Dependence of mechanical properties of polyamide components on build parameters in the SLS process
(Elsevier, 2007)Customised properties of parts manufactured using the selective laser sintering process are achievable by variation of build parameters. The energy density, controlled by laser power, distance between scan lines and speed ... -
Engineering assisted surgery: A route for digital design and manufacturing of customized maxillofacial implants
(Elsevier, 2007)Rapid prototyping (RP) and more recently rapid manufacturing (RM) as a part of Engineering Assisted Surgery enables manufacturing of customised implants and prostheses prior to surgical procedures. Beginning with CT ...