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Specificity and sensitivity evaluation of novel and existing Bacteroidales and Bifidobacteria-specific PCR assays on feces and sewage samples and their application for microbial source tracking in Ireland

ARAN - Access to Research at NUI Galway

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dc.contributor.author Dorai-Raj, Siobhan en
dc.contributor.author O' Grady, Justin en
dc.contributor.author Colleran, Emer en
dc.date.accessioned 2010-03-12T14:59:19Z en
dc.date.available 2010-03-12T14:59:19Z en
dc.date.issued 2009 en
dc.identifier.citation Dorai-Raj, S., Grady, J. O., & Colleran, E. (2009). Specificity and sensitivity evaluation of novel and existing Bacteroidales and Bifidobacteria-specific PCR assays on feces and sewage samples and their application for microbial source tracking in Ireland. Water Research, 43(19), 4980-4988. en
dc.identifier.uri http://hdl.handle.net/10379/782 en
dc.description.abstract Three novel ruminant-specific PCR assays, an existing ruminant-specific PCR assay and five existing human-specific PCR assays, which target 16S rDNA from Bacteroidales or Bifidobacteria, were evaluated. The assays were tested on DNA extracted from ruminant (n = 74), human (n = 59) and non-ruminant animal (n = 44) sewage/fecal samples collected in Ireland. The three novel PCR assays compared favourably to the existing ruminant-specific assay, exhibiting sensitivities of 91 - 100% and specificities of 95 - 100% as compared to a sensitivity of 95% and specificity of 94%, for the existing ruminant-specific assay. Of the five human-specific PCR assays, the assay targeting the Bifidobacterium catenulatum group was the most promising, exhibiting a sensitivity of 100% (with human sewage samples) and a specificity of 87%. When tested on rural water samples that were naturally contaminated by ruminant feces, the three novel PCR assays tested positive with a much greater percentage (52 - 87%) of samples than the existing ruminant-specific assay (17%). These novel ruminant-specific assays show promise for microbial source tracking and merit further field testing and specificity evaluation. en
dc.description.sponsorship ERTDI en
dc.format application/pdf en
dc.language.iso en en
dc.publisher Elsevier en
dc.subject Bifidobacteria en
dc.subject Bacteroidales en
dc.subject Microbial source tracking en
dc.subject Fecal pollution en
dc.subject PCR en
dc.subject.lcsh Foodborne diseases en
dc.subject.lcsh Microbial toxins en
dc.subject.lcsh Pathogenic microorganisms -- Detection en
dc.subject.lcsh Diagnostic microbiology. en
dc.title Specificity and sensitivity evaluation of novel and existing Bacteroidales and Bifidobacteria-specific PCR assays on feces and sewage samples and their application for microbial source tracking in Ireland en
dc.type Article en
dc.local.publishedsource http://dx.doi.org/10.1016/j.watres.2009.08.050 en
dc.description.peer-reviewed peer-reviewed en

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