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Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector

ARAN - Access to Research at NUI Galway

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dc.contributor.author Mathew, Asha
dc.contributor.author Cao, Hongliang
dc.contributor.author Collin, Estelle
dc.contributor.author Wang, Wenxin
dc.contributor.author Pandit, Abhay
dc.date.accessioned 2012-10-24T09:52:05Z
dc.date.available 2012-10-24T09:52:05Z
dc.date.issued 2012-06-02
dc.identifier.citation Mathew, A,Cao, HL,Collin, E,Wang, WX,Pandit, A (2012) 'Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector'. International Journal Of Pharmaceutics, 434 :99-105. en_US
dc.identifier.issn 0378-5173
dc.identifier.uri http://hdl.handle.net/10379/3004
dc.description.abstract A unique hyperbranched polymeric system with a linear poly-2-dimethylaminoethyl methacrylate (pDMAEMA) block and a hyperbranched polyethylene glycol methyl ether methacrylate (PEGMEMA) and ethylene dimethacrylate (EGDMA) block was designed and synthesized via deactivation enhanced atom transfer radical polymerisation (DE-ATRP) for efficient gene delivery. Using this unique structure, with a linear pDMAEMA block, which efficiently binds to plasmid DNA (pDNA) and hyperbranched polyethylene glycol (PEG) based block as a protective shell, we were able to maintain high transfection levels without sacrificing cellular viability even at high doses. The transfection capability and cytotoxicity of the polymers over a range of pDNA concentration were analysed and the results were compared to commercially available transfection vectors such as polyethylene imine (branched PEI, 25 kDa), partially degraded poly(amido amine) dendrimer (dPAMAM; commercial name: SuperFect (R)) in fibroblasts and adipose tissue derived stem cells (ADSCs). (c) 2012 Elsevier B.V. All rights reserved. en_US
dc.format application/pdf en_US
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.relation.ispartof International Journal Of Pharmaceutics en
dc.subject 2-(Dimethylamino) ethyl methacrylate en_US
dc.subject Deactivation enhanced atom transfer radical polymerisation en_US
dc.subject Gene therapy en_US
dc.title Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector en_US
dc.type Article en_US
dc.date.updated 2012-10-18T16:26:34Z
dc.identifier.doi DOI 10.1016/j.ijpharm.2012.05.010
dc.description.peer-reviewed peer-reviewed
dc.contributor.funder |~|
dc.internal.rssid 2208128
dc.local.contact Abhay Shashikant Pandit, Mechanical & Biomedical Eng, College Of Eng & Informatics, Room 304, Nfb Building, Ida Bus Park, Dangan, Nui Galway. 2758 Email: abhay.pandit@oegaillimh.ie
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