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A stainless steel multi-well plate (SS-MWP) for high-throughput Raman analysis of dilute solutions

ARAN - Access to Research at NUI Galway

Show simple item record Ryder, Alan G. Li, Boyan Ryan, Paul W. Sirimuthu, Narayana M. S. 2012-09-28T18:28:23Z 2012-09-28T18:28:23Z 2010
dc.identifier.citation Ryder, A.G., De Vincentis, J., Li, B., Ryan, P.W., Sirimuthu, N.M.S., Leister ,K.J. (2010) 'A stainless steel multi-well plate (SS-MWP) for high-throughput Raman analysis of dilute solutions'. Journal Of Raman Spectroscopy, 41 (10):1266-1275. en_US
dc.identifier.issn 1097-4555
dc.description Journal article en_US
dc.description.abstract The use of Raman spectroscopy for the qualitative and quantitative analysis of dilute aqueous solutions is of interest to the biopharmaceutical manufacturing sector. However, the inherent weakness of the Raman effect, coupled with spectral variability due to spurious signals from sample holders, can produce significant problems for chemometric-based high-throughput assays. Therefore, there is a need for a multi-well sample holder that ensures robust and repeatable measurements, in particular from dilute aqueous solutions such as cell culture media. Here we demonstrate the efficacy of a novel, electropolished, stainless steel multi-well plate (SS-MWP) sample holder with 96 wells for dilute aqueous solution analysis. A comprehensive study of the spectroscopic behaviour was carried out and comparisons made with multi-well plates fabricated from polystyrene, polypropylene, and aluminium. A key factor in the validation studies is the use of intrinsically weak Raman scattering systems, e.g. water and dilute glucose solutions. The data collected show that the SS-MWPs are much superior in terms of robustness, resistance to chemical attack, and measurement reproducibility and as such are the ideal sample holders for Raman analysis of dilute solutions. en_US
dc.description.sponsorship Bristol-Myers Squibb; Industrial Development Authority (IDA) Centre for Bioanalytical Sciences (CBAS) Programme en_US
dc.format application/pdf en_US
dc.language.iso en en_US
dc.publisher Wiley en_US
dc.relation.ispartof Journal Of Raman Spectroscopy en
dc.subject Raman spectroscopy en_US
dc.subject Sample holder en_US
dc.subject Similarity analysis en_US
dc.subject Squeous solution en_US
dc.subject High throughput en_US
dc.subject Spectroscopy en_US
dc.title A stainless steel multi-well plate (SS-MWP) for high-throughput Raman analysis of dilute solutions en_US
dc.type Article en_US 2012-08-17T15:10:22Z
dc.identifier.doi DOI 10.1002/jrs.2586
dc.local.publishedsource en_US
dc.description.peer-reviewed peer-reviewed
dc.contributor.funder |~|
dc.internal.rssid 1159059 Alan Ryder, School Of Chemistry, Room 228, Arts/Science Building, Nui Galway. 2943 Email:
dc.local.copyrightchecked No
dc.local.version ACCEPTED

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