dc.contributor.author | Li, Boyan | |
dc.contributor.author | Ryan, Paul W. | |
dc.contributor.author | Ray, Bryan H. | |
dc.contributor.author | Sirimuthu, Narayana M. S. | |
dc.contributor.author | Ryder, Alan G. | |
dc.date.accessioned | 2012-08-23T11:20:15Z | |
dc.date.available | 2012-08-23T11:20:15Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Li B., Ryan P.W., Ray B.H., Leister K.J., Sirimuthu N.M.S., Ryder A.G. (2010) 'Rapid characterization and quality control of complex cell culture media solutions using Raman spectroscopy and chemometrics'. Biotechnology And Bioengineering, 107 (2):290-301. | en_US |
dc.identifier.issn | 1097-0290 | |
dc.identifier.uri | http://hdl.handle.net/10379/2942 | |
dc.description | Journal article | en_US |
dc.description.abstract | The use of Raman spectroscopy coupled with chemometrics for the rapid identification, characterization, and quality assessment of Complex cell culture media components used for industrial mammalian cell culture was investigated. Raman spectroscopy offers significant advantages for the analysis of complex, aqueous-based materials used in biotechnology because there is no need for sample preparation and water is a weak Raman scatterer. We demonstrate the efficacy of the method for the routine analysis of dilute aqueous solution of five different chemically defined (CD) commercial media components used in a Chinese Hamster Ovary (CHO) cell manufacturing process for recombinant proteins. The chemometric processing of the Raman spectral data is the key factor in developing robust methods. Here, we discuss the optimum methods for eliminating baseline drift, background fluctuations, and other instrumentation artifacts to generate reproducible spectral data. Principal component analysis (PCA) and soft independent modeling of class analogy (SIMCA) were then employed in the development of a robust routine for both identification and quality evaluation of the five different media components. These methods have the potential to be extremely useful in an industrial context for "in-house"; sample handling, tracking, and quality control. Biotechnol. Bioeng. 2010;107: 290-301. (c) 2010 Wiley Periodicals, Inc. | en_US |
dc.description.sponsorship | Industrial Development Authority; Bristol¿Myers Squibb [Centre for Bioanalytical Sciences] | en_US |
dc.format | application/pdf | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Biotechnology And Bioengineering | en |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | Raman spectroscopy | en_US |
dc.subject | Chemometrics | en_US |
dc.subject | Cell culture | en_US |
dc.subject | Chemically defined media | en_US |
dc.subject | PCA | en_US |
dc.subject | SIMCA | en_US |
dc.subject | Principal component analysis | en_US |
dc.subject | Near-infrared spectroscopy | en_US |
dc.subject | Solid mixtures | en_US |
dc.subject | Biomedical applications | en_US |
dc.subject | Biopsy specimens | en_US |
dc.subject | Cereal foods | en_US |
dc.subject | Serum-free | en_US |
dc.subject | Classification | en_US |
dc.title | Rapid characterization and quality control of complex cell culture media solutions using Raman spectroscopy and chemometrics | en_US |
dc.type | Article | en_US |
dc.date.updated | 2012-08-17T14:05:38Z | |
dc.identifier.doi | DOI 10.1002/bit.22813 | |
dc.local.publishedsource | http://dx.doi.org/10.1002/bit.22813 | en_US |
dc.description.peer-reviewed | peer-reviewed | |
dc.contributor.funder | |~|Other|~| | |
dc.internal.rssid | 1159083 | |
dc.local.contact | Alan Ryder, School Of Chemistry, Room 228, Arts/Science Building, Nui Galway. 2943 Email: alan.ryder@nuigalway.ie | |
dc.local.copyrightchecked | Yes | |
dc.local.version | ACCEPTED | |
nui.item.downloads | 1962 | |