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Fluorescence EEM Spectroscopy for Rapid Identification and Quality Evaluation of Cell Culture Media Components.

ARAN - Access to Research at NUI Galway

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dc.contributor.author Li, Boyan
dc.contributor.author Ryan, Paul W.
dc.contributor.author Shanahan, Michael
dc.contributor.author Ryder, Alan G.
dc.date.accessioned 2012-08-23T11:15:45Z
dc.date.available 2012-08-23T11:15:45Z
dc.date.issued 2011-11
dc.identifier.citation B. Li, P.W. Ryan, M. Shanahan, K.J. Leister, and A.G. Ryder (2011) 'Fluorescence EEM Spectroscopy for Rapid Identification and Quality Evaluation of Cell Culture Media Components'. Applied Spectroscopy, 65 (11):1240-1249. en_US
dc.identifier.issn 1943-3530
dc.identifier.uri http://hdl.handle.net/10379/2941
dc.description Journal article. en_US
dc.description.abstract The application of fluorescence excitation-emission matrix (EEM) spectroscopy to the quantitative analysis of complex, aqueous solutions of cell culture media components was investigated.  These components, yeastolate, phytone, recombinant human insulin, eRDF basal medium, and four different chemically defined (CD) media, are used for the formulation of basal and feed media employed in the production of recombinant proteins using a Chinese Hamster Ovary (CHO) cell based process.  The comprehensive analysis (either identification or quality assessment) of these materials using chromatographic methods is time consuming and expensive, and not suitable for high throughput quality control.  The use of EEM in conjunction with multi-way chemometric methods provides for a rapid, non-destructive analytical method suitable for the screening of large numbers of samples.   Here we use multiway robust principal component analysis (MROBPCA) in conjunction with N-way partial least-squares-discriminant analysis (NPLS-DA) to develop a robust routine for both the identification and quality evaluation of these important cell culture materials:  These methods are applicable to a wide range of complex mixtures since they do not rely on any predetermined compositional or property information, thus making them potentially very useful for sample handling, tracking, and quality assessment in biopharmaceutical industries.  en_US
dc.description.sponsorship Industrial Development Authority; Bristol-Myers Squibb [Centre for BioAnalytical Sciences] en_US
dc.format application/pdf en_US
dc.language.iso en en_US
dc.publisher Ingenta en_US
dc.relation.ispartof Applied Spectroscopy en
dc.subject Fluorescence, en_US
dc.subject EEM, en_US
dc.subject Cell culture media, en_US
dc.subject Chemometrics en_US
dc.title Fluorescence EEM Spectroscopy for Rapid Identification and Quality Evaluation of Cell Culture Media Components. en_US
dc.type Article en_US
dc.date.updated 2012-08-17T14:16:26Z
dc.identifier.doi http://dx.doi.org/10.1366/11-06383
dc.local.publishedsource http://dx.doi.org/10.1366/11-06383 en_US
dc.description.peer-reviewed peer-reviewed
dc.contributor.funder |~|Industrial Contracts|~|
dc.internal.rssid 1284880
dc.local.contact Alan Ryder, School Of Chemistry, Room 228, Arts/Science Building, Nui Galway. 2943 Email: alan.ryder@nuigalway.ie
dc.local.copyrightchecked Yes
dc.local.version ACCEPTED

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