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Prediction of Cell Culture Media performance using Fluorescence Spectroscopy

ARAN - Access to Research at NUI Galway

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dc.contributor.author Ryan, Paul W.
dc.contributor.author Li, Boyan
dc.contributor.author Shanahan, Michael
dc.contributor.author Ryder, Alan G.
dc.date.accessioned 2012-08-22T11:03:54Z
dc.date.available 2012-08-22T11:03:54Z
dc.date.issued 2010
dc.identifier.citation Ryan, P.W., Li, B., Shanahan, M., Leister, K.J., Ryder ,A.G. (2010) 'Prediction of Cell Culture Media performance using Fluorescence Spectroscopy'. Analytical Chemistry, 82 :1311-1317. en_US
dc.identifier.issn 1520-6882
dc.identifier.uri http://www.nuigalway.ie/nanoscale/publications.html
dc.identifier.uri http://hdl.handle.net/10379/2932
dc.description Journal article en_US
dc.description.abstract Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in efficient manufacturing. We are exploring the use of rapid holistic analytical methods that can be used for routine screening of cell culture media used in industrial biotechnology.  The application of rapid fluorescence spectroscopic techniques to the routine analysis of cell culture media (Chinese hamster ovary cell-based manufacture) was investigated. We have developed robust methods which can be used to identify compositional changes and ultimately predict the efficacy of individual fed batchmedia in terms of downstream protein product yield with an accuracy of (0.13 g/L. This is achieved through the implementation of chemometric methods such as multiway robust principal component analysis (MROBPCA), and n-way partial least-squares-discriminant analysis and regression (NPLS-DA and NPLS). This ability to observe compositional changes and predict product yield before media use has enormous potential and should permit the effective elimination of one of the major process variables leading to more consistent product quality and improved yield. These robust and reliable methods have the potential to become an important part of upstream biopharmaceutical quality control and analysis. en_US
dc.description.sponsorship Irish Industrial Development Authority; Bristol-Myers Squibb; en_US
dc.format application/pdf en_US
dc.language.iso en en_US
dc.publisher ACS Publications en_US
dc.relation.ispartof Analytical Chemistry en
dc.subject Biopharmaceutical en_US
dc.subject Fluorescence en_US
dc.subject Chemometrics en_US
dc.subject quantitative en_US
dc.subject protein en_US
dc.title Prediction of Cell Culture Media performance using Fluorescence Spectroscopy en_US
dc.type Article en_US
dc.date.updated 2012-08-17T14:00:25Z
dc.identifier.doi http://dx.doi.org/10.1021/ac902337c
dc.local.publishedsource http://dx.doi.org/10.1021/ac902337c en_US
dc.description.peer-reviewed peer-reviewed
dc.contributor.funder |~|Other|~|
dc.internal.rssid 1158820
dc.local.contact Alan Ryder, School Of Chemistry, Room 228, Arts/Science Building, Nui Galway. 2943 Email: alan.ryder@nuigalway.ie
dc.local.copyrightchecked Yes
dc.local.version ACCEPTED

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