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Evaluation of acridine in Nafion as a fluorescence-lifetime-based pH sensor

ARAN - Access to Research at NUI Galway

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dc.contributor.author Ryder, Alan G.
dc.contributor.author Power, Sarah
dc.contributor.author Glynn, Thomas J.
dc.date.accessioned 2012-08-22T09:59:43Z
dc.date.available 2012-08-22T09:59:43Z
dc.date.issued 2003
dc.identifier.citation Ryder, AG,Power, S,Glynn, TJ (2003) 'Evaluation of acridine in Nafion as a fluorescence-lifetime-based pH sensor'. Applied Spectroscopy, 57 (1):73-79. en_US
dc.identifier.issn 1943-3530
dc.identifier.uri http://www.opticsinfobase.org/as/abstract.cfm?URI=as-57-1-73
dc.identifier.uri http://hdl.handle.net/10379/2928
dc.description Journal article en_US
dc.description.abstract We report a novel fluorescence-lifetime-based pH sensing method that utilizes acridine incorporated into Nation (AcNaf) as the fluorescent indicator. The AcNaf sensor is excited using a 380 nm light emitting diode (LED) and the fluorescence lifetimes are measured at 450 and 500 nm. The fluorescence behavior of acridine as a function of pH in aqueous phosphate buffers and incorporated into the Nation membrane has been investigated. The results show that incorporating acridine into Nation changes the apparent ground-state pK(a) from similar to5.45 to similar to9, while the apparent excited-state pK(a)* is only slightly changed (similar to9.4 in 0.1 M phosphate buffer). The AcNaf film shows a good pH response with a change in average lifetime of similar to19 ns (at an emission wavelength of 450 nm) over the pH 8 to 10 range. We also show that excited-state protonation does not occur in the AcNaf sensor film and that chloride quenching cannot occur because of the permselective nature of Nafion. We also discuss how the unique structure of Nation affects the fluorescence behavior of acridine at various pH values and examine the impact of buffer concentration on apparent pK(a) and pH sensing ability. en_US
dc.description.sponsorship NUI Galway Millennium Research Grant; Enterprise Ireland Research Innovation Fund (IF/2001/061); HEA Programme for Research en_US
dc.format application/pdf en_US
dc.language.iso en en_US
dc.relation.ispartof Applied Spectroscopy en
dc.subject Fluorescence lifetime en_US
dc.subject Acridine en_US
dc.subject Sensor en_US
dc.subject Nafion en_US
dc.subject Ultraviolet en_US
dc.subject Light emitting diode en_US
dc.subject pH en_US
dc.subject Time-resolved fluorescence en_US
dc.subject Light-emitting diode en_US
dc.subject Phase-modulation fluorometry en_US
dc.subject Optical sensors en_US
dc.subject Silica-gel en_US
dc.subject Decay time en_US
dc.subject Nanosecond en_US
dc.subject Luminescence en_US
dc.subject State en_US
dc.subject Absorption en_US
dc.title Evaluation of acridine in Nafion as a fluorescence-lifetime-based pH sensor en_US
dc.type Article en_US
dc.date.updated 2012-08-17T12:55:16Z
dc.local.publishedsource http://www.opticsinfobase.org/as/abstract.cfm?URI=as-57-1-73 en_US
dc.description.peer-reviewed peer-reviewed
dc.contributor.funder |~|
dc.internal.rssid 1346951
dc.local.contact Alan Ryder, School Of Chemistry, Room 228, Arts/Science Building, Nui Galway. 2943 Email: alan.ryder@nuigalway.ie
dc.local.copyrightchecked No
dc.local.version ACCEPTED

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