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Cell cycle-dependent mobility of Cdc45 in living cells determined by fluorescence correlation spectroscopy.

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dc.contributor.author Broderick, Ronan
dc.contributor.author Ramadurai, Sivaramakrishnan
dc.contributor.author Togashi, Denisio M.
dc.contributor.author Ryder, Alan G.
dc.contributor.author Langowski, Jorg
dc.date.accessioned 2012-07-16T10:02:25Z
dc.date.available 2012-07-16T10:02:25Z
dc.date.issued 2012
dc.identifier.citation R. Broderick, S. Ramadurai, K. Toth, D. Togashi, A. G. Ryder, J. Langwoski, and H.P. Nasheuer. (2012) 'Cell cycle-dependent mobility of Cdc45 in living cells determined by fluorescence correlation spectroscopy'. Plos One, 7 (4):e35537-e35537. en_US
dc.identifier.issn 1932-6203
dc.identifier.uri http://www.nuigalway.ie/nanoscale/publications.html
dc.identifier.uri http://hdl.handle.net/10379/2905
dc.description.abstract Eukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS) in vivo in asynchronous cells and in cells synchronized at the G1/S transition and during S phase. Our data show that eGFP-Cdc45 mobility is faster in G1/S transition compared to S phase suggesting that Cdc45 is part of larger protein complex formed in S phase. Furthermore, the size of complexes containing Cdc45 was estimated in asynchronous, G1/S and S phase-synchronized cells using gel filtration chromatography; these findings complemented the in vivo FCS data. Analysis of the mobility of eGFP-Cdc45 and the size ofcomplexes containing Cdc45 and eGFP-Cdc45 after UVC-mediated DNA damage revealed no significant changes in diffusion rates and complex sizes using FCS and gel filtration chromatography analyses. This suggests that after UV-damage, Cdc45 is still present in a large multi-protein complex and that its mobility within living cells is consistently similar following UVC-mediated DNA damage. en_US
dc.format application/pdf en_US
dc.language.iso en en_US
dc.publisher Public Library of Science en_US
dc.relation.ispartof Plos One en
dc.subject Eukaryotic DNA replication
dc.title Cell cycle-dependent mobility of Cdc45 in living cells determined by fluorescence correlation spectroscopy. en_US
dc.type Article en_US
dc.date.updated 2012-07-09T12:36:44Z
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0035537
dc.local.publishedsource http://dx.doi.org/10.1371/journal.pone.0035537 en_US
dc.description.peer-reviewed peer-reviewed
dc.contributor.funder |~|SFI|~|
dc.internal.rssid 1662726
dc.local.contact Alan Ryder, School Of Chemistry, Room 228, Arts/Science Building, Nui Galway. 2943 Email: alan.ryder@nuigalway.ie
dc.local.copyrightchecked Yes
dc.local.version ACCEPTED

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