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Functional dissection of the Smc5/6 complex: roles of the SUMO ligase Nse2/Mms21 in the maintenance of genome stability

ARAN - Access to Research at NUI Galway

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dc.contributor.advisor Morrison, Ciaran
dc.contributor.author Kliszczak, Maciej
dc.date.accessioned 2012-06-14T11:57:23Z
dc.date.available 2013-01-21T13:10:03Z
dc.date.issued 2011-06
dc.identifier.uri http://hdl.handle.net/10379/2861
dc.description.abstract Nse2/ Mms21 is an E3 SUMO ligase component of the Smc5/6 complex, which plays multiple roles in maintaining genome stability. To study the functions of the vertebrate Nse2 orthologue, we generated Nse2-deficient chicken DT40 cells. Nse2 was dispensable for DT40 cell viability and required for efficient repair of bulky DNA lesions, although double-strand break repair was unaffected. Homologous recombination activities were reduced in Nse2-/-/- cells. Nse2 deficiency destabilised Smc5, but not Smc6. In rescue experiments, we found that the SUMO ligase activity of Nse2 was required for an efficient response to MMS-induced DNA damage, but not for Smc5 stability. Analysis of Nse2-/-/- Smc5- clones, which were viable although slow-growing, showed that Smc5 and Nse2 are not epistatic in DNA repair, suggesting that Smc5 and Nse2 have some non-overlapping functions. en_US
dc.subject SMC proteins, en_US
dc.subject Nse2 en_US
dc.subject Mms21 en_US
dc.subject DNA repair en_US
dc.subject DT40 en_US
dc.subject SUMO ligase en_US
dc.subject Biochemistry en_US
dc.title Functional dissection of the Smc5/6 complex: roles of the SUMO ligase Nse2/Mms21 in the maintenance of genome stability en_US
dc.type Thesis en_US
dc.contributor.funder College of Science Felllowship en_US
dc.local.note Maciej Kliszczak has graduated from Wroclaw University of Technology with Engineer and Master of Science degrees in Biotechnology (Medicinal Chemistry) in 2007, defending thesis entitled "Responses of human cell lines to DNA damaging agents and to novel cancer growth inhibitors". In 2007, Maciej Kliszczak moved to Galway and started his Doctor of Philosophy Degree at National University of Ireland Galway under the guidance of Prof. Ciaran Morrison and defended his dissertation thesis in 2011. en_US
dc.local.final Yes en_US

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