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Novel Multiplex Real-Time PCR Diagnostic Assay for Identification and Differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis Complex Strains

ARAN - Access to Research at NUI Galway

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dc.contributor.author Reddington, Kate
dc.contributor.author O'Grady, Justin
dc.contributor.author Dorai-Raj, Siobhan
dc.contributor.author Maher, Majella
dc.contributor.author van Soolingen, Dick
dc.contributor.author Barry, Thomas
dc.date.accessioned 2012-02-13T13:44:30Z
dc.date.available 2012-02-13T13:44:30Z
dc.date.issued 2011-02
dc.identifier.citation Reddington, K., J. O'Grady, S. Dorai-Raj, M. Maher, D. van Soolingen, and T. Barry. 2011. Novel Multiplex Real-Time PCR Diagnostic Assay for Identification and Differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis Complex Strains. J. Clin. Microbiol. 49:651-657 en_US
dc.identifier.uri http://hdl.handle.net/10379/2563
dc.description.abstract Tuberculosis (TB) in humans is caused by members of the Mycobacterium tuberculosis complex (MTC). Rapid detection of the MTC is necessary for the timely initiation of antibiotic treatment, while differentiation between members of the complex may be important to guide the appropriate antibiotic treatment and provide epidemiological information. In this study, a multiplex real-time PCR diagnostics assay using novel molecular targets was designed to identify the MTC while simultaneously differentiating between M. tuberculosis and M. canettii. The lepA gene was targeted for the detection of members of the MTC, the wbbl1 gene was used for the differentiation of M. tuberculosis and M. canettii from the remainder of the complex, and a unique region of the M. canettii genome, a possible novel region of difference (RD), was targeted for the specific identification of M. canettii. The multiplex real-time PCR assay was tested using 125 bacterial strains (64 MTC isolates, 44 nontuberculosis mycobacteria [NTM], and 17 other bacteria). The assay was determined to be 100% specific for the mycobacteria tested. Limits of detection of 2.2, 2.17, and 0.73 cell equivalents were determined for M. tuberculosis/M. canettii, the MTC, and M. canettii, respectively, using probit regression analysis. Further validation of this diagnostics assay, using clinical samples, should demonstrate its potential for the rapid, accurate, and sensitive diagnosis of TB caused by M. tuberculosis, M. canettii, and the other members of the MTC. en_US
dc.format doi:10.1128/JCM.01426-10 en_US
dc.language.iso en en_US
dc.publisher Journal of Clinical Microbiology en_US
dc.subject Tuberculosis en_US
dc.subject Mycobacterium tuberculosis complex (MTC) en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject Mycobacterium canettii en_US
dc.subject Microbiology en_US
dc.title Novel Multiplex Real-Time PCR Diagnostic Assay for Identification and Differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis Complex Strains en_US
dc.type Article en_US
dc.local.publishedsource http://jcm.asm.org/content/49/2/651.full.pdf+html en_US
dc.description.peer-reviewed peer-reviewed en_US
dc.contributor.funder College of Sciences, National University of Ireland Galway funded KR Postgraduate Scholarship en_US

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