ARAN - Access to Research at NUI Galway

Fear-induced suppression of nociceptive behaviour and activation of signal transduction molecules in the rat periaqueductal grey: role of fatty acid amide hydrolase.

ARAN - Access to Research at NUI Galway

Show simple item record

dc.contributor.author Butler, Ryan K.
dc.contributor.author Ford, Gemma K.
dc.contributor.author Hogan, Michelle
dc.contributor.author Roche, Michelle
dc.contributor.author Doyle, Karen M.
dc.contributor.author Kelly, John P.
dc.contributor.author Finn, David P.
dc.date.accessioned 2012-01-20T10:54:16Z
dc.date.available 2012-11-21T15:57:07Z
dc.date.issued 2011-09-17
dc.identifier.citation Butler RK, Ford GK, Hogan M, Roche M, Doyle KM, Kelly JP, Kendall DA, Chapman V, Finn DP (2011). Fear-induced suppression of nociceptive behaviour and activation of signal transduction molecules in the rat periaqueductal grey: role of fatty acid amide hydrolase. en_US
dc.identifier.uri http://hdl.handle.net/10379/2497
dc.description.abstract The endocannabinoid system regulates nociception and aversion and mediates fear-conditioned analgesia (FCA). We investigated the effects of the fatty acid amide hydrolase (FAAH) inhibitor URB597, which inhibits the catabolism of the endocannabinoid anandamide and related N-acylethanolamines, on expression of FCA and fear and pain related behaviour per se in rats. We also examined associated alterations in the expression of the signal transduction molecule phospho-Akt in the periaqueductal grey (PAG) by immunoblotting. FCA was modelled by assessing formalin-evoked nociceptive behaviour in an arena previously paired with footshock. URB597 (0.3 mg/kg, i.p.) enhanced FCA and increased fear-related behaviour in formalin-treated rats. Conditioned fear per se in non-formalin-treated rats was associated with increased expression of phospho-Akt in the PAG. URB597 reduced the expression of fear-related behaviour in the early part of the trial, an effect that was accompanied by attenuation of the fear-induced increase in phospho-Akt expression in the PAG. Intra-plantar injection of formalin also reduced the fear-induced increase in phospho-Akt expression. These data provide evidence for a role of FAAH in FCA, fear responding in the presence or absence of nociceptive tone, and fear-evoked increases in PAG phospho-Akt expression. In addition, the results suggest that fear-evoked activation of Akt signalling in the PAG is abolished in the presence of nociceptive tone. en_US
dc.format application/pdf en_US
dc.language.iso en en_US
dc.publisher Sage en_US
dc.subject Akt (protein kinase B) en_US
dc.subject Endocannabinoids en_US
dc.subject Fatty acid amide hydrolase (FAAH) en_US
dc.subject Fear en_US
dc.subject Pain en_US
dc.subject Periaqueductal grey (PAG) en_US
dc.subject Rats en_US
dc.subject Pharmacology &Therapeutics en_US
dc.title Fear-induced suppression of nociceptive behaviour and activation of signal transduction molecules in the rat periaqueductal grey: role of fatty acid amide hydrolase. en_US
dc.type Article en_US
dc.local.publishedsource http://dx.doi.org/10.1177/0269881111413823 en_US
dc.description.peer-reviewed peer-reviewed en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record