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Rapid characterization and quality control of complex cell culture media solutions using raman spectroscopy and chemometrics

ARAN - Access to Research at NUI Galway

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dc.contributor.author Li, Boyan
dc.contributor.author Ryan, Paul W.
dc.contributor.author Ray, Bryan H.
dc.contributor.author Sirimuthu, Narayana M.S.
dc.contributor.author Ryder, Alan G.
dc.date.accessioned 2011-11-11T16:26:52Z
dc.date.available 2011-11-11T16:26:52Z
dc.date.issued 2010
dc.identifier.citation B. Li, P.W. Ryan, B.H. Ray, K.J. Leister, N.M.S. Sirimuthu, and A.G. Ryder (2010) Rapid Characterisation and Quality Control of Complex Cell Culture Media Solutions using Raman Spectroscopy and Chemometrics. Biotechnology and Bioengineering, 107(2), 290-301 en_US
dc.identifier.uri http://hdl.handle.net/10379/2313
dc.description.abstract The use of Raman spectroscopy coupled with chemometrics for the rapid identification, characterization, and quality assessment of complex cell culture media components used for industrial mammalian cell culture was investigated. Raman spectroscopy offers significant advantages for the analysis of complex, aqueous-based materials used in biotechnology because there is no need for sample preparation and water is a weak Raman scatterer. We demonstrate the efficacy of the method for the routine analysis of dilute aqueous solution of five different chemically defined (CD) commercial media components used in a Chinese Hamster Ovary (CHO) cell manufacturing process for recombinant proteins.The chemometric processing of the Raman spectral data is the key factor in developing robust methods. Here, we discuss the optimum methods for eliminating baseline drift, background fluctuations, and other instrumentation artifacts to generate reproducible spectral data. Principal component analysis (PCA) and soft independent modeling of class analogy (SIMCA) were then employed in the development of a robust routine for both identification and quality evaluation of the five different media components. These methods have the potential to be extremely useful in an industrial context for 'in-house' sample handling, tracking, and quality control. Biotechnol. Bioeng. 2010;107: 290-301. © 2010 Wiley Periodicals, Inc. en_US
dc.format application/pdf en_US
dc.language.iso en en_US
dc.subject Chemistry en_US
dc.subject Raman spectroscopy en_US
dc.subject Chemometrics en_US
dc.subject Cell culture en_US
dc.subject Chemically defined media en_US
dc.subject PCA en_US
dc.subject SIMCA en_US
dc.title Rapid characterization and quality control of complex cell culture media solutions using raman spectroscopy and chemometrics en_US
dc.type Article en_US
dc.local.publishedsource http://dx.doi.org/10.1002/bit.22813 en_US
dc.description.peer-reviewed peer-reviewed en_US

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