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Controlling dispersion of axonal regeneration using a multichannel collagen nerve conduit

ARAN - Access to Research at NUI Galway

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dc.contributor.author Yao, Li en
dc.contributor.author Pandit, Abhay en
dc.date.accessioned 2011-02-18T09:56:05Z en
dc.date.available 2011-02-18T09:56:05Z en
dc.date.issued 2010-03-21 en
dc.identifier.citation Yao, L., de Ruiter, G. C. W., Wang, H., Knight, A. M., Spinner, R. J., Yaszemski, M. J., et al. Controlling dispersion of axonal regeneration using a multichannel collagen nerve conduit. Biomaterials, 31(22), 5789-5797. en
dc.identifier.issn 0142-9612 en
dc.identifier.uri http://hdl.handle.net/10379/1619 en
dc.description.abstract Single channel conduits are used clinically in nerve repair as an alternative to the autologous nerve graft. Axons regenerating across single channel tubes, however, may disperse resulting in inappropriate target reinnervation. This dispersion may be limited by multichannel nerve conduits as they resemble the structure of nerve multiple basal lamina tubes. In this study, we investigated the influence of channel number on the axonal regeneration using a series of 1-, 2-, 4-, and 7-channel collagen conduits and commercial (NeuraGen®) single channel conduits. Nerve conduits were implanted in rats with a 1 cm gap of sciatic nerve. After four months, quantitative results of regeneration were evaluated with nerve morphometry and the accuracy of regeneration was assessed using retrograde tracing: two tracers being applied simultaneously to tibial and peroneal nerves to determine the percentage of motor neurons with double projections. Recovery of function was investigated with compound muscle action potential recordings and ankle motion analysis. We showed that the fabricated 1-channel and 4-channel conduits are superior to other types of conduits in axonal regeneration. Simultaneous tracing showed a significantly lower percentage of motor neurons with double projections after 2- and 4-channel compared with 1-channel conduit repair. This study shows the potential influence of multichannel guidance on limiting dispersion without decreasing quantitative results of regeneration. en
dc.format application/pdf en
dc.language.iso en en
dc.publisher Elsevier en
dc.subject Collagen en
dc.subject Nerve regeneration en
dc.subject Nerve conduit en
dc.subject Neural tissue engineering en
dc.subject In vivo en
dc.subject Network of Excellence for Functional Biomaterials en
dc.title Controlling dispersion of axonal regeneration using a multichannel collagen nerve conduit en
dc.type Article en
dc.local.publishedsource http://dx.doi.org/10.1016/j.biomaterials.2010.03.081 en
dc.description.peer-reviewed peer-reviewed en
dc.contributor.funder Enterprise Ireland - Proof of Concept Grant (PC/2007/027) en
dc.contributor.funder Science Foundation Ireland - Research Frontiers Program, under Grant Number (08/RFP/ENM1218) en
dc.contributor.funder Science Foundation Ireland - The Short Term Travel Fellowship (STTF) supplement en
dc.contributor.funder Leiden University Fund (LUF)/ Slingelands en

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