Browsing University of Galway Theses (PhD Theses) by Subject "DNA damage response"
Now showing items 1-13 of 13
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Centriolar and ciliary responses to DNA damage
(2012-09-26)Centrosome duplication is tightly controlled and takes place only once every cell cycle. In recent years more light has been shed on the mechanism of centrosome duplication, with key players being identified and characterised. ... -
Characterisation of novel Histone H3 RAD9 mutants defective in the DNA damage response in Saccharomyces cerevisiae
(2013-02-28)The DNA damage response (DDR) is a signal transduction cascade, which regulates cell cycle progression, gene transcription and DNA repair. Histones also play a vital role in the activation of the DDR and have been shown ... -
Deciphering the role of human MSL2 and MSL1 within and beyond the MSL complex
(2018-01-14)While the components of the MSL (Male Specific Lethal) complex in Drosophila are well studied and known to play a critical role in dosage compensation, there is little known about the function of its evolutionary conserved ... -
Effects of proteasome inhibition on cisplatin induced DNA damage responses in human cells
(2012-02-07)The proteasome is the main site of protein degradation in human cells, and plays a major role in the regulation of cellular processes including cell cycle progression, DNA repair and the DNA damage response. Proteasome ... -
Functional Analysis of Vertebrate Msl2
(2014-02-12)hMSL2 (Male Specific Lethal 2, human) is a RING finger protein with E3 ubiquitin ligase activity. Although it has been shown to target histone H2B at lysine 34 and p53 at lysine 351, suggesting roles in transcription ... -
Implications of histone H2AX abundance in breast cancer
(NUI Galway, 2019-12-09)Histones are responsible for the packaging of DNA into a eukaryotic cell nucleus, and the H2A variant H2AX plays an important role in the DNA damage response (DDR). The phosphorylated form of H2AX, known as γH2AX, has been ... -
The kinesin KIF18B interacts with 53BP1 and is required for efficient double strand break repair
(2015-11-19)Genetic changes can lead to detrimental genomic instability, mutagenesis, oncogenesis and premature aging. The DNA damage response (DDR) is responsible for safeguarding the genome from such harmful alterations. 53BP1 ... -
The microprocessing factor DGCR8 interacts with ATM and functions independently of its partner DROSHA in the DDR
(NUI Galway, 2020-01-23)The eukaryotic cell has evolved a complex suite of mechanisms called the DNA damage response (DDR), that acts by sensing DNA damage and reacting appropriately to maintain genomic integrity. The PIK kinase ATM is a key ... -
Regulation and function of the tumour suppressor 53BP1 at sites of DNA damage
(2013-10-16)Cancer is the major cause of death for people in middle age. It results from cell transformation into malignant cells and propagates with normal controls. This process is induced by mutations occurring in DNA through the ... -
Reverse genetic analysis of centrosomal roles of Checkpoint Kinase 1
(NUI Galway, 2019-06-14)Centrosome duplication is tightly regulated process that occurs only once per cell cycle. Centrosome abnormalities have been observed in cancer cells and in cells with defective DNA damage responses. Genotoxic stresses ... -
Reverse Genetic Analysis of Pericentrin Functions
(2012-06-11)The centrosome is a subcellular organelle that organises the mitotic spindle microtubules to ensure accurate segregation of chromosomes during cell division. Most animal centrosomes comprise a pair of centrioles which are ... -
RNA metabolism proteins identified as ATM and 53BP1 partners function in the DDR
(2017-07-05)The DNA is continually under treat of being damaged by both endogenous and exogenous sources such as reactive oxygen species produced during normal cell metabolism or ionising radiation. It is vital for the maintenance of ... -
Roles of DNA polymerase eta and replication protein A (RPA) in undamaged and platinum-treated human cells
(2012-09-28)Platinum-based drugs are widely used in cancer therapy. Bypass of platinum-induced DNA adducts during DNA replication by specialised DNA polymerases may contribute to drug tolerance and tumour cell resistance. Human cells ...