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The influence of size and charge of chitosan/polyglutamic acid hollow spheres on cellular internalization, viability and blood compatibility

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dc.contributor.author Dash, Biraja C. en
dc.contributor.author Réthoré, Gildas en
dc.contributor.author Monaghan, Michael en
dc.contributor.author Pandit, Abhay en
dc.date.accessioned 2010-10-14T09:31:02Z en
dc.date.available 2010-10-14T09:31:02Z en
dc.date.issued 2010-11 en
dc.identifier.citation Dash, B. C., Réthoré, G., Monaghan, M., Fitzgerald, K., Gallagher, W., & Pandit, A. The influence of size and charge of chitosan/polyglutamic acid hollow spheres on cellular internalization, viability and blood compatibility. Biomaterials, 31(32), 8188-8197. en
dc.identifier.issn 0142-9612 en
dc.identifier.uri http://hdl.handle.net/10379/1339 en
dc.description.abstract Polymeric hollow spheres can be tailored as efficient carriers of various therapeutic molecules due to their tunable properties. However, the entry of these synthetic vehicles into cells, their cell viability and blood compatibility depend on their physical and chemical properties e.g. size, surface charge. Herein, we report the effect of size and surface charge on cell viability and cellular internalization behaviour and their effect on various blood components using chitosan/polyglutamic acid hollow spheres as a model system. Negatively charged chitosan/polyglutamic acid hollow spheres of various sizes 100, 300, 500 and 1000 nm were fabricated using a template based method and covalently surface modified using linear polyethylene glycol and methoxyethanol amine to create a gradient of surface charge from negative to neutrally charged spheres respectively. The results here suggest that both size and surface charge have a significant influence on the sphere¿s behaviour, most prominently on haemolysis, platelet activation, plasma recalcification time, cell viability and internalization over time. Additionally, cellular internalization behaviour and viability was found to vary with different cell types. These results are in agreement with those of inorganic spheres and liposomes, and can serve as guidelines for tailoring polymeric solid spheres for specific desired applications in biological and pharmaceutical fields, including the design of nanometer to submicron-sized delivery vehicles. en
dc.format application/pdf en
dc.publisher Elsevier en
dc.subject Size en
dc.subject Surface charge en
dc.subject Cell viability en
dc.subject Internalization en
dc.subject Blood compatibility en
dc.subject Nanospheres en
dc.subject Microspheres en
dc.subject Network of Excellence for Functional Biomaterials en
dc.title The influence of size and charge of chitosan/polyglutamic acid hollow spheres on cellular internalization, viability and blood compatibility en
dc.type Article en
dc.local.publishedsource http://www.sciencedirect.com/science/article/B6TWB-50RMPNR-4/2/03d2b7dcb4165a75593ac3f36d8628f6 en
dc.description.peer-reviewed peer-reviewed en

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