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Type II collagen-hyaluronan hydrogel - a step towards a scaffold for intervertebral disc tissue engineering

ARAN - Access to Research at NUI Galway

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dc.contributor.author Calderon, Laura en
dc.contributor.author Collin, Estelle en
dc.contributor.author Murphy, Mary en
dc.contributor.author O'Halloran, Damien en
dc.contributor.author Pandit, Abhay en
dc.date.accessioned 2010-10-14T09:13:38Z en
dc.date.available 2010-10-14T09:13:38Z en
dc.date.issued 2010-09-06 en
dc.identifier.citation Calderon, L., Collin, E., Velasco-Bayon, D., Murphy, M., O'Halloran, D., & Pandit, A. (2010). Type II collagen-hyaluronan hydrogel ¿ a step towards a scaffold for intervertebral disc tissue engineering. "European Cells & Materials", 20, 134-148 en
dc.identifier.issn 1473-2262 en
dc.identifier.uri http://hdl.handle.net/10379/1334 en
dc.description.abstract Intervertebral disc regeneration strategies based on stem cell differentiation in combination with the design of functional scaffolds is an attractive approach towards repairing/regenerating the nucleus pulposus. The specific aim of this study was to optimise a composite hydrogel composed of type II collagen and hyaluronic acid (HA) as a carrier for mesenchymal stem cells. Hydrogel stabilisation was achieved by means of 1-ethyl-3(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) cross-linking. Optimal hydrogel properties were determined by investigating different concentrations of EDC (8 mM, 24 mM and 48 mM). Stable hydrogels were obtained independent of the concentration of carbodiimide used. The hydrogels cross-linked by the lowest concentration of EDC (8 mM) demonstrated high swelling properties. Additionally, improved proliferation of seeded rat mesenchymal stem cells (rMSCs) and hydrogel stability levels in culture were observed with this 8 mM cross-linked hydrogel. Results from this study indicate that EDC/NHS (8 mM) cross-linked type II collagen/HA hydrogel was capable of supporting viability of rMSCs, and furthermore their differentiation into a chondrogenic lineage. Further investigations should be conducted to determine its potential as scaffold for nucleus pulposus regeneration/repair. en
dc.format application/pdf en
dc.language.iso en_US en
dc.publisher European Cells & Materials Ltd en
dc.subject Intervertebral disc regeneration en
dc.subject Network of Excellence for Functional Biomaterials en
dc.title Type II collagen-hyaluronan hydrogel - a step towards a scaffold for intervertebral disc tissue engineering en
dc.type Article en
dc.local.publishedsource http://www.ecmjournal.org/journal/papers/vol020/vol020a12.php en
dc.description.peer-reviewed peer-reviewed en
dc.contributor.funder Science Foundation of Ireland, Research Frontiers Programme en

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