Browsing School of Chemistry (Scholarly Articles) by Subject "Fluorescence"
Now showing items 1-20 of 22
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Accurate anisotropy recovery from fluorophore mixtures using Multivariate Curve Resolution (MCR)
(Elsevier, 2017-11-22)Anisotropy resolved multidimensional emission spectroscopy (ARMES) provides valuable insights into multi-fluorophore systems like proteins that have complex overlapping emission bands. The method combines multidimensional ... -
Analysis of hydrocarbon bearing fluid inclusions HCFI) using time-resolved fluorescence spectroscopy
(Society of Photo-optical Instrumentation Engineers, 2005)Hydrocarbon-bearing fluid inclusions (HCFI) are microscopic cavities within rocks that are filled with petroleum oil, the composition of which may not have changed since the trapping event. Thus, the composition of that ... -
Characterization of Lysozyme PEGylation products using polarized Excitation Emission Matrix (pEEM) spectroscopy
(Wiley, 2020-07-07)The growing use of therapeutic proteins requires accurate measurement techniques for measuring biophysical and structural changes during manufacturing. This is particularly true for PEGylation of proteins, because ... -
Evaluating the interaction of human serum albumin (HSA) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes in different aqueous environments using anisotropy resolved multi-dimensional emission spectroscopy (ARMES)
(Elsevier, 2022-01-07)Studying the interaction between plasma proteins and liposomes is critical, particularly for their use as drug delivery systems. Here, the efficacy of anisotropy resolved multidimensional emission spectroscopy (ARMES) for ... -
A fluorescence anisotropy method for measuring protein concentration in complex cell culture media
(Elsevier, 2014-03-12)The rapid, quantitative analysis of the complex cell culture media used in biopharmaceutical manufacturing is of critical importance. Requirements for cell culture media composition profiling, or changes in specific analyte ... -
Fluorescence emission from petroleum; a valuable information source for petroleum analysis.
(EniTecnologie, 2004)Despite the fact that most crude oils are dark coloured liquids, many are observed to be strongly fluorescent when illuminated with UV light. This fluorescence, which originates from the presence of aromatic compounds, is ... -
Frequency domain fluorescence lifetime study of crude petroleum oils
(Springer Verlag, 2008-02-01)Frequency domain (FD) fluorescence lifetime data was collected for a series of 20 crude petroleum oils using a 405 nm excitation source and over a spectral range of similar to 426 to similar to 650 nm. Average fluorescence ... -
Hydrocarbon fluid inclusion fluorescence: A review
(Springer, 2007)Geological fluid inclusions are small voids that can contain a variety of liquids which are often found in natural minerals and rocks. Typically they are less than 10 micrometres in size that host fossil fluids which existed ... -
Influence of chemical composition on the fluorescence lifetimes of crude petroleum oils
(Society of Photo-optical Instrumentation Engineers, 2003)Fluorescence based methods are increasingly being used for the analysis of crude oils because they offer high speed, low cost, non-contact, and non-destructive testing options. The fluorescence of crude oils is due to the ... -
Investigating native state fluorescence emission of Immunoglobulin G using polarized Excitation Emission Matrix (pEEM) spectroscopy and PARAFAC
(Elsevier, 2018-12-20)Intrinsic fluorescence spectroscopy (IFS) measurements for protein structural analysis can be enhanced by the use of anisotropy resolved multidimensional emission spectroscopy (ARMES). ARMES attempts to overcome the ... -
Modelling Förster resonance energy transfer (FRET) using anisotropy resolved multi-dimensional emission spectroscopy (ARMES)
(Elsevier, 2020-11-16)Background Förster Resonance Energy Transfer (FRET) is widely used to study the structure and dynamics of biomolecular systems and also causes the non-linear fluorescence response observed in multi-fluorophore proteins. ... -
Monitoring local unfolding of bovine serum albumin during denaturation using steady-state and time-resolved fluorescence spectroscopy
(Springer, 2010-03)In a previous report (J. Fluoresc. 16, 153, 2006) we studied the chaotropiclly induced denaturation of Bovine Serum Albumin (BSA) using the fluorescence decay kinetics at different stages in the denaturation of BSA by ... -
Multi-attribute quality screening of immunoglobulin G using polarized Excitation Emission Matrix spectroscopy
(Elsevier, 2019-12-10)Immunoglobulin G (IgG) is often used as a starting material for the production of functionalised antibodies, like Antibody Drug Conjugates (ADCs), PEGlyated-conjugates, or radioimmunoconjugates. The gross structural quality ... -
Prediction of Cell Culture Media Performance Using Fluorescence Spectroscopy
(2010)Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in ... -
Prediction of Cell Culture Media performance using Fluorescence Spectroscopy
(ACS Publications, 2010)Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in ... -
Prediction of cell culture media performance using fluorescence spectroscopy.
(American Chemical Society, 2010)Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in ... -
A rapid fluorescence based method for the quantitative analysis of cell culture media photo-degradation
(Elsevier (Science Direct), 2013-11-21)Cell culture media are very complex chemical mixtures that are one of the most important aspects in biopharmaceutical manufacturing. The complex composition of many media leads to materials that are inherently unstable and ... -
Rapid quantification of tryptophan and tyrosine in chemically defined cell culture media using fluorescence spectroscopy
(2012)The rapid and inexpensive analysis of the complex cell culture media used in industrial mammalian cell culture is required for quality and variance monitoring. Excitation-emission matrix (EEM) spectroscopy combined with ... -
Study of water adsorption in PolyN-isopropylacrylamide) thin films using fluorescence emission of 3-hydroxyflavone probes
(ACS Publications, 2010)The noncontact measurement of water uptake in microscale , thermoresponsive poly(N-isopropylacrylamide) thin films is challenging. We assessed the efficacy of three different 3-hydroxyflavone (3-HF)-based fluorophores to ... -
Super stable fluorescein isothiocyanate isomer I monolayer for total internal reflection fluorescence microscopy
(American Chemical Society, 2018-08-27)Total internal reflection fluorescence microscopy (TIRFM) is an important method in surface science and for the analysis of surface-bound macromolecules. Here, we developed and explored the use of a novel fluorescein ...