Browsing School of Chemistry by Subject "Fluorescence"
Now showing items 1-20 of 26
-
Accurate anisotropy recovery from fluorophore mixtures using Multivariate Curve Resolution (MCR)
(Elsevier, 2017-11-22)Anisotropy resolved multidimensional emission spectroscopy (ARMES) provides valuable insights into multi-fluorophore systems like proteins that have complex overlapping emission bands. The method combines multidimensional ... -
Analysis of crude petroleum oils using fluorescence spectroscopy.
(Springer, 2005)Crude oil is defined as a mixture of hydrocarbons that existed in the liquid phase in natural underground reservoirs and remains liquid at atmospheric pressure after passing through surface separating facilities (joint ... -
Analysis of hydrocarbon bearing fluid inclusions HCFI) using time-resolved fluorescence spectroscopy
(Society of Photo-optical Instrumentation Engineers, 2005)Hydrocarbon-bearing fluid inclusions (HCFI) are microscopic cavities within rocks that are filled with petroleum oil, the composition of which may not have changed since the trapping event. Thus, the composition of that ... -
Anisotropy resolved multidimensional emission spectroscopy (ARMES): A new tool for protein analysis
(Elsevier, 2015-07-30)Structural analysis of proteins using the emission of intrinsic fluorophores is complicated by spectral overlap. Anisotropy resolved multidimensional emission spectroscopy (ARMES) overcame the overlap problem by the use ... -
Characterization of Lysozyme PEGylation products using polarized Excitation Emission Matrix (pEEM) spectroscopy
(Wiley, 2020-07-07)The growing use of therapeutic proteins requires accurate measurement techniques for measuring biophysical and structural changes during manufacturing. This is particularly true for PEGylation of proteins, because ... -
Evaluating the interaction of human serum albumin (HSA) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes in different aqueous environments using anisotropy resolved multi-dimensional emission spectroscopy (ARMES)
(Elsevier, 2022-01-07)Studying the interaction between plasma proteins and liposomes is critical, particularly for their use as drug delivery systems. Here, the efficacy of anisotropy resolved multidimensional emission spectroscopy (ARMES) for ... -
A fluorescence anisotropy method for measuring protein concentration in complex cell culture media
(Elsevier, 2014-03-12)The rapid, quantitative analysis of the complex cell culture media used in biopharmaceutical manufacturing is of critical importance. Requirements for cell culture media composition profiling, or changes in specific analyte ... -
Fluorescence emission from petroleum; a valuable information source for petroleum analysis.
(EniTecnologie, 2004)Despite the fact that most crude oils are dark coloured liquids, many are observed to be strongly fluorescent when illuminated with UV light. This fluorescence, which originates from the presence of aromatic compounds, is ... -
Fluorescence lifetime based pH sensing using Resorufin
(2003)Accurate, non-contact pH sensing is of particular importance in the biological and clinical sciences. Fluorescence lifetime based pH sensing is potentially more useful than intensity based methods because of the reduced ... -
Fluorescence study of bovine serum albumin and Ti and Sn Oxide nanoparticles interactions.
(The Optical Society, 2007)Nanochemistry offers stimulating opportunities for a wide variety of applications in the biosciences. Understanding of the interaction of nanoparticles with biomolecules such as proteins is very important as it can help ... -
Frequency domain fluorescence lifetime study of crude petroleum oils
(Springer Verlag, 2008-02-01)Frequency domain (FD) fluorescence lifetime data was collected for a series of 20 crude petroleum oils using a 405 nm excitation source and over a spectral range of similar to 426 to similar to 650 nm. Average fluorescence ... -
Hydrocarbon fluid inclusion fluorescence: A review
(Springer, 2007)Geological fluid inclusions are small voids that can contain a variety of liquids which are often found in natural minerals and rocks. Typically they are less than 10 micrometres in size that host fossil fluids which existed ... -
Influence of chemical composition on the fluorescence lifetimes of crude petroleum oils
(Society of Photo-optical Instrumentation Engineers, 2003)Fluorescence based methods are increasingly being used for the analysis of crude oils because they offer high speed, low cost, non-contact, and non-destructive testing options. The fluorescence of crude oils is due to the ... -
Investigating native state fluorescence emission of Immunoglobulin G using polarized Excitation Emission Matrix (pEEM) spectroscopy and PARAFAC
(Elsevier, 2018-12-20)Intrinsic fluorescence spectroscopy (IFS) measurements for protein structural analysis can be enhanced by the use of anisotropy resolved multidimensional emission spectroscopy (ARMES). ARMES attempts to overcome the ... -
Modelling Förster resonance energy transfer (FRET) using anisotropy resolved multi-dimensional emission spectroscopy (ARMES)
(Elsevier, 2020-11-16)Background Förster Resonance Energy Transfer (FRET) is widely used to study the structure and dynamics of biomolecular systems and also causes the non-linear fluorescence response observed in multi-fluorophore proteins. ... -
Monitoring local unfolding of bovine serum albumin during denaturation using steady-state and time-resolved fluorescence spectroscopy
(Springer, 2010-03)In a previous report (J. Fluoresc. 16, 153, 2006) we studied the chaotropiclly induced denaturation of Bovine Serum Albumin (BSA) using the fluorescence decay kinetics at different stages in the denaturation of BSA by ... -
Multi-attribute quality screening of immunoglobulin G using polarized Excitation Emission Matrix spectroscopy
(Elsevier, 2019-12-10)Immunoglobulin G (IgG) is often used as a starting material for the production of functionalised antibodies, like Antibody Drug Conjugates (ADCs), PEGlyated-conjugates, or radioimmunoconjugates. The gross structural quality ... -
Prediction of Cell Culture Media Performance Using Fluorescence Spectroscopy
(2010)Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in ... -
Prediction of Cell Culture Media performance using Fluorescence Spectroscopy
(ACS Publications, 2010)Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in ... -
Prediction of cell culture media performance using fluorescence spectroscopy.
(American Chemical Society, 2010)Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in ...