Browsing by Author "Santocanale, Corrado"
Now showing items 1-20 of 24
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A high through-put screen for small molecules modulating mcm2 phosphorylation identifies ryuvidine as an inducer of the dna damage response
FitzGerald, Jennifer; Murillo, Laura S.; O'Brien, Gemma; O'Connell, Enda; O'Connor, Aisling; Wu, Kevin; Wang, Guan-Nan; Rainey, Michael D.; Natoni, Alessandro; Healy, Sandra; O'Dwyer, Michael; Santocanale, Corrado (Public Library of Science (PLoS), 2014-06-05)DNA replication is an essential process for cell division and as such it is a process that is directly targeted by several anticancer drugs. CDC7 plays an essential role in the activation of replication origins and has ... -
CDC7 kinase promotes replication DNA double-strand breaks upon replication fork stalling and its inhibition causes an inflammatory response and senescence
Cazzaniga, Chiara (NUI Galway, 2022-12-08)CDC7 is an essential serine-threonine kinase that has an important role in the initiation of DNA replication. CDC7 phosphorylates several sites of the MCM helicase complex allowing recruitment of CDC45, among other factors, ... -
CDC7 kinase regulates DNA replication fork processing and its inhibition is modulated by PTBP1
Quinlan, Aisling (NUI Galway, 2022-09-21)CDC7 is an essential serine/threonine kinase that regulates the initiation of DNA replication by phosphorylating several sites of the MCM helicase complex. Several inhibitors of CDC7 are available that reduce cell proliferation ... -
Cdc7-dependent and -independent phosphorylation of claspin in the induction of the dna replication checkpoint
Rainey, Michael; Harhen, Brendan; Wang, Guan-Nan; Murphy, Paul; Santocanale, Corrado (Informa UK Limited, 2013-05-15)Claspin is a critical mediator protein in the DNA replication checkpoint, responsible for ATR-dependent activation of the effector kinase Chk1. Cdc7, an essential kinase required for the initiation of DNA replication, can ... -
Cell cycle-dependent formation of Cdc45-Claspin complexes in human cells is compromized by UV-mediated DNA damage
Broderick, Ronan; Rainey, Michael D.; Santocanale, Corrado; Nasheuer, Heinz-Peter (Wiley, 2013-08-02)The replication factor Cdc45 has essential functions in the initiation and elongation steps of eukaryotic DNA replication and plays an important role in the intra-S-phase checkpoint. Its interactions with other replication ... -
Characterisation of the cellular responses to CDC7 inhibition in breast-derived human cells
Quach Thi Thu, Huong (2017-10-06)CDC7 plays a role in DNA replication initiation and cell cycle regulation. Inhibition of CDC7 kinase by siRNA triggers a p53-independent apoptotic cell death in cancer cells but not in human fibroblast suggesting this ... -
Ddk dependent regulation of top2a at centromeres revealed by a chemical genetics approach
Wu, Kevin Z. L.; Wang, Guan-Nan; Fitzgerald, Jennifer; Quachthithu, Huong; Rainey, Michael D.; Cattaneo, Angela; Bachi, Angela; Santocanale, Corrado (Oxford University Press (OUP), 2016-07-12)In eukaryotic cells the CDC7/DBF4 kinase, also known as DBF4-dependent kinase (DDK), is required for the firing of DNA replication origins. CDC7 is also involved in replication stress responses and its depletion sensitises ... -
DNA mediated chromatin pull-down for the study of chromatin replication
Kliszczak, Anna E.; Rainey, Michael D.; Harhen, Brendan; Santocanale, Corrado (2011)Chromatin replication involves duplicating DNA while maintaining epigenetic information. These processes are critical for genome stability and for preserving cell-type identity. Here we describe a simple experimental ... -
Dna mediated chromatin pull-down for the study of chromatin replication
Kliszczak, Anna E.; Rainey, Michael D.; Harhen, Brendan; Boisvert, Francois M.; Santocanale, Corrado (Springer Nature, 2011-09-19)Chromatin replication involves duplicating DNA while maintaining epigenetic information. These processes are critical for genome stability and for preserving cell-type identity. Here we describe a simple experimental ... -
DNA mediated chromatin pull-down: a novel method for the study of chromatin replication
Kliszczak, Anna Ewelina (2012-04-27)Chromatin replication involves duplicating DNA while maintaining epigenetic information. These processes are critical for genome stability and for preserving cell-type identity. To investigate how these processes are ... -
Evidence for the progression through s-phase in the ectopic cell cycle re-entry of neurons in alzheimer disease
Bonda, David J.; Evans, Teresa A.; Santocanale, Corrado; Llosá, Jesús Catalá; Viňa, Jose; Bajic, Vladan P.; Castellani, Rudy J.; Siedlak, Sandra L.; Perry, George; Smith, Mark A.; Lee, Hyoung-gon (Impact Journals, LLC, 2009-04-23)Aberrant neuronal re-entry into the cell cycle is emerging as a potential pathological mechanism in Alzheimer disease (AD). However, while cyclins, cyclin dependent kinases (CDKs), and other mitotic factors are ectopically ... -
Examining a new class of dual CDC7/CDK9 inhibitors, mechanisms of proliferation, and proliferation-based stratification in myeloma
Coyne, Mark Robert (2014-09-05)A key feature of progressive myeloma is a relative increase in proliferation. The DBF4-dependent kinase (DDK), cell division cycle 7 (CDC7), is an essential kinase for initiation of DNA replication. This thesis assesses ... -
Identification of IRS4 and TOP2A as novel CDC7 kinase interacting proteins
Wu, Kevin Zhi Loong (2016-02-08)CDC7 is an essential serine/threonine kinase required for the initiation of DNA replication in eukaryotic cells, through phosphorylating the MCM helicase complex. Formation of an active kinase requires the binding to ... -
Investigating the roles of Chl1 helicase in DNA damage tolerance pathways
Reyes, Teresa Anne Clarisse (NUI Galway, 2023-01-09)Faithful DNA replication is fundamental to ensure proper genomic stability. Eukaryotic cells have evolved sophisticated networks to deal with DNA lesions encountered during chromosome replication. The DNA damage response ... -
Kinase Inhibitor against Quiescent and Proliferating CLL Cells
Natoni, Alessandro; Murill, Laura S.; Kliszczak, Anna E.; Samali, Afshin; Santocanale, Corrado (2011-07-18)In chronic lymphocytic leukemia (CLL) the proliferation rate and resistance to drug-induced apoptosis are recognized as important factors in the outcome of treatment. In this study, we assess the activity and the mechanism ... -
Life with crippled microtubules: How yeast and human cells deal with forced microtubule depolymerization
Pavani, Mattia (NUI Galway, 2023-01-25)Microtubule poisons are microtubule targeting agents widely used in cancer treatment. These drugs are given to cancer patients in cycles, and resistant cells can emerge both after the first or the following rounds of ... -
Maintenance of the spindle assembly checkpoint by PLK1 and CDC7 kinases and characterisation of cell lines carrying mutations in the genes coding for the CDC7 regulatory subunits
O'Connor, Aisling (2016-11-15)The Spindle Assembly Checkpoint (SAC) ensures that accurate chromosome segregation occurs, by preventing progression into anaphase until all kinetochore-microtubule attachments are stable and bi-polar. During a mitotic ... -
Requirement for PLK1 kinase activity in the maintenance of a robust spindle assembly checkpoint
Maffini, Stefano; Rainey, Michael D.; Kaczmarczyk, Agnieszka; O'Connor, Aisling; Gaboriau, David; Musacchio, Andrea; Santocanale, Corrado (Company of Biologists, 2016)During mitotic arrest induced by microtubule targeting drugs, the weakening of the spindle assembly checkpoint (SAC) allows cells to progress through the cell cycle without chromosome segregation occurring. PLK1 kinase ... -
A role for USP9X in homologous recombination repair of DNA double strand breaks
O'Dea, Rachel (NUI Galway, 2020-02-11)In order to prevent the deleterious effects of a variety of genotoxic agents, cells have developed complex surveillance mechanisms and multiple DNA repair pathways that allow them to maintain genome integrity. The ... -
Synthesis of migrastatin analogues as inhibitors of tumour cell migration: Exploring structural change in and on the macrocyclic ring
Lo Re, Daniele; Zhou, Ying; Mucha, Joanna; Jones, Leigh F.; Leahy, Lorraine; Santocanale, Corrado; Krol, Magdalena; Murphy, Paul V. (Wiley, 2015-11-04)Migrastatin and isomigrastatin analogues have been synthesised in order to contribute to structure-activity studies on tumour cell migration inhibitors. These include macrocycles varying in ring size, functionality and ...