Browsing by Author "Rainey, Michael D."
Now showing items 1-7 of 7
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A high through-put screen for small molecules modulating mcm2 phosphorylation identifies ryuvidine as an inducer of the dna damage response
FitzGerald, Jennifer; Murillo, Laura S.; O'Brien, Gemma; O'Connell, Enda; O'Connor, Aisling; Wu, Kevin; Wang, Guan-Nan; Rainey, Michael D.; Natoni, Alessandro; Healy, Sandra; O'Dwyer, Michael; Santocanale, Corrado (Public Library of Science (PLoS), 2014-06-05)DNA replication is an essential process for cell division and as such it is a process that is directly targeted by several anticancer drugs. CDC7 plays an essential role in the activation of replication origins and has ... -
Cell cycle-dependent formation of Cdc45-Claspin complexes in human cells is compromized by UV-mediated DNA damage
Broderick, Ronan; Rainey, Michael D.; Santocanale, Corrado; Nasheuer, Heinz-Peter (Wiley, 2013-08-02)The replication factor Cdc45 has essential functions in the initiation and elongation steps of eukaryotic DNA replication and plays an important role in the intra-S-phase checkpoint. Its interactions with other replication ... -
Ddk dependent regulation of top2a at centromeres revealed by a chemical genetics approach
Wu, Kevin Z. L.; Wang, Guan-Nan; Fitzgerald, Jennifer; Quachthithu, Huong; Rainey, Michael D.; Cattaneo, Angela; Bachi, Angela; Santocanale, Corrado (Oxford University Press (OUP), 2016-07-12)In eukaryotic cells the CDC7/DBF4 kinase, also known as DBF4-dependent kinase (DDK), is required for the firing of DNA replication origins. CDC7 is also involved in replication stress responses and its depletion sensitises ... -
DNA mediated chromatin pull-down for the study of chromatin replication
Kliszczak, Anna E.; Rainey, Michael D.; Harhen, Brendan; Santocanale, Corrado (2011)Chromatin replication involves duplicating DNA while maintaining epigenetic information. These processes are critical for genome stability and for preserving cell-type identity. Here we describe a simple experimental ... -
Dna mediated chromatin pull-down for the study of chromatin replication
Kliszczak, Anna E.; Rainey, Michael D.; Harhen, Brendan; Boisvert, Francois M.; Santocanale, Corrado (Springer Nature, 2011-09-19)Chromatin replication involves duplicating DNA while maintaining epigenetic information. These processes are critical for genome stability and for preserving cell-type identity. Here we describe a simple experimental ... -
Requirement for PLK1 kinase activity in the maintenance of a robust spindle assembly checkpoint
Maffini, Stefano; Rainey, Michael D.; Kaczmarczyk, Agnieszka; O'Connor, Aisling; Gaboriau, David; Musacchio, Andrea; Santocanale, Corrado (Company of Biologists, 2016)During mitotic arrest induced by microtubule targeting drugs, the weakening of the spindle assembly checkpoint (SAC) allows cells to progress through the cell cycle without chromosome segregation occurring. PLK1 kinase ... -
The deubiquitinase usp9x maintains dna replication fork stability and dna damage checkpoint responses by regulating claspin during s-phase
McGarry, Edel; Gaboriau, David; Rainey, Michael D.; Restuccia, Umberto; Bachi, Angela; Santocanale, Corrado (American Association for Cancer Research (AACR), 2016-02-26)