Browsing by Author "Kane, Marian"
Now showing items 1-11 of 11
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Campylobacter jejuni strain discrimination and temperature-dependent glycome expression profiling by lectin microarray
Kilcoyne, Michelle; Twomey, Marcus E.; Gerlach, Jared Q.; Kane, Marian; Moran, Anthony P.; Joshi, Lokesh (Elsevier, 2014-02-12)Gram-negative Campylobacter jejuni is the leading cause of bacterial gastroenteritis in humans worldwide and the most frequently identified infectious trigger in patients developing Guillain-Barré syndrome (GBS). While ... -
Characterization of human melanoma cell lines and melanocytes by proteome analysis
Caputo, Emilia; Maiorana, Luigi; Vasta, Valeria; Pezzino, Franca M.; Sunkara, Sudhir; Wynne, Kieran; Elia, Giuliano; Marincola, Francesco M.; McCubrey, James A.; Libra, Massimo; Travali, Salvatore; Kane, Marian (Informa UK Limited, 2011-09-01)We have analyzed the proteomes of two human melanoma cell lines (A375 and 526), and of the human melanocytes, (FOM 78), by two-dimensional electrophoresis (2D-PAGE) and liquid chromatography-tandem mass spectrometry ... -
Construction of a Natural Mucin Microarray and Interrogation for Biologically Relevant Glyco-Epitopes
Kilcoyne, Michelle; Gerlach, Jared Q.; Kane, Marian; Joshi, Lokesh (2012)Mucins are the principal components of mucus, and mucin glycosylation has important roles in defense, microbial adhesion, immunomodulation, inflammation, and cancer. Mucin expression and glycosylation are dynamic, responding ... -
Cross-feeding by bifidobacterium breve ucc2003 during co-cultivation with bifidobacterium bifidum prl2010 in a mucin-based medium
Egan, Muireann; O’Connell Motherway, Mary; Kilcoyne, Michelle; Kane, Marian; Joshi, Lokesh; Ventura, Marco; van Sinderen, Douwe (Springer Nature, 2014-11-25)Background: Bifidobacteria constitute a specific group of commensal bacteria that commonly inhabit the mammalian gastrointestinal tract. Bifidobacterium breve UCC2003 was previously shown to utilize a variety of ... -
Differential release of high mannose structural isoforms by fungal and bacterial endo-β-N-acetylglucosaminidases
Gerlach, Jared Q.; Kilcoyne, Michelle; Farrell, Mark P.; Kane, Marian; Joshi, Lokesh (Royal Society of Chemistry, 2012-02-28)Endo-beta-N-acetylglucosaminidases (ENGases) are widely used to remove N-linked oligosaccharides from glycoproteins for glycomic and proteomic studies and biopharmaceutical processes. Although several ENGases are widely ... -
Exposure of bifidobacterium longum subsp. infantis to milk oligosaccharides increases adhesion to epithelial cells and induces a substantial transcriptional response
Kavanaugh, Devon W.; O’Callaghan, John; Buttó, Ludovica F.; Slattery, Helen; Lane, Jonathan; Clyne, Marguerite; Kane, Marian; Joshi, Lokesh; Hickey, Rita M. (Public Library of Science (PLoS), 2013-06-21)In this study, we tested the hypothesis that milk oligosaccharides may contribute not only to selective growth of bifidobacteria, but also to their specific adhesive ability. Human milk oligosaccharides (3'sialyllactose ... -
Generation of a panel of high affinity antibodies and development of a biosensor-based immunoassay for the detection of okadaic acid in shellfish
Le Berre, Marie; Kilcoyne, Michelle; Kane, Marian (Elsevier, 2015-07-10)Okadaic acid (OA) and its derivatives, DTX-1 and DTX-2, are marine biotoxins associated with diarrhetic shellfish poisoning. Routine monitoring of these toxins relies on the mouse bioassay. However, due to the technical ... -
Glycosylation-related gene expression in ht29-mtx-e12 cells upon infection by helicobacter pylori
Cairns, Michael T; Gupta, Ananya; Naughton, Julie A; Kane, Marian; Clyne, Marguerite; Joshi, Lokesh (Baishideng Publishing Group Inc., 2017-10-07)AIM To identify glycosylation-related genes in the HT29 derivative cell line, HT29-MTX-E12, showing differential expression on infection with Helicobacter pylori (H. pylori). METHODS Polarised HT29-MTX-E12 cells were ... -
Identification of putative adhesins and carbohydrate ligands of Lactobacillus paracasei using a combinatorial in silico and glycomics microarray profiling approach
Houeix, Benoit; Synowsky, Silvia; Cairns, Michael T.; Kane, Marian; Kilcoyne, Michelle; Joshi, Lokesh (Oxford University Press (OUP), 2019-11-11)Commensal bacteria must colonize host mucosal surfaces to exert health-promoting properties, and bind to gastrointestinal tract (GIT) mucins via their cell surface adhesins. Considerable effort has been directed towards ... -
Rapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray
Utratna, Marta; Annuk, Heidi; Gerlach, Jared Q.; Lee, Yuan C.; Kane, Marian; Kilcoyne, Michelle; Joshi, Lokesh (Nature Research, 2017-07-25)There is an urgent need for discovery of novel antimicrobials and carbohydrate-based anti-adhesive strategies are desirable as they may not promote resistance. Discovery of novel anti-adhesive molecules from natural product ... -
Surface chemistry and linker effects on lectin-carbohydrate recognition for glycan microarrays
Kilcoyne, Michelle; Gerlach, Jared Q.; Kane, Marian; Joshi, Lokesh (2012)Glycan microarrays are an increasingly utilised tool for analysis of protein-carbohydrate interactions and a variety of glycan-containing molecules and slide chemistries have been used to array carbohydrates on microarray ...