Browsing by Subject "Glycosylation"
Now showing items 1-17 of 17
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Construction of a Natural Mucin Microarray and Interrogation for Biologically Relevant Glyco-Epitopes
(2012)Mucins are the principal components of mucus, and mucin glycosylation has important roles in defense, microbial adhesion, immunomodulation, inflammation, and cancer. Mucin expression and glycosylation are dynamic, responding ... -
Development and characterisation of recognition molecules for the specific identification of non-human glycans
(2015-01-26)Glycosylation is an enzymatic process through which glycans attach to proteins, lipids and other organic molecules with huge structural diversity. This is a common post-translational modification found on proteins and cell ... -
Exploring the role of glycosylation in vascular biology, immunology,and stem cell biology
(2016-09-30)Carbohydrates are important modulators in various biological and physiological processes. They are present ubiquitously and coat all cells, forming the glycocalyx of the cell. Glycosylation, a type of post-translational ... -
A glyco approach for the treatment of Parkinson’s disease: From the glycobiological characterisation to the development of glyco-modulatory collagen-based hydrogel systems
(NUI Galway, 2021-12-20)Parkinson’s disease (PD) is the most common motor-related progressive neurodegenerative condition, mainly caused by the loss of dopaminergic neurons in the substantia nigra. Despite the increased knowledge regarding its ... -
Glycosylation and glycoengineering of recombinant antibody fragments in Escherichia coli
(2016-11-14)Recombinant protein production (RPP) in Escherichia coli benefits from a long history of research which has facilitated the development of a plethora of strains and vectors suited to RPP applications. The identification ... -
Glycosylation in the injured and regenerating spinal cord, and the influence of biomaterial treatment
(NUI Galway, 2020-02-04)Traumatic spinal cord injury (SCI) results in disruption of tissue integrity leading to a loss of function. There has been minimal success in treating these injuries clinically, due to the inhibitory environment which ... -
Lectin microarray profiling demonstrates equivalent global glycosylation for whey protein ingredients enriched with α-lactalbumin and milk fat globule membrane
(Elsevier, 2022-12-22)Human milk fat globule membrane (MFGM) and whey proteins are nutritionally and functionally valuable, with many beneficial bioactivities associated with their glycosylation. However glycosylation of milk components other ... -
Lectins: getting familiar with translators of the sugar code
(MDPI, 2015-01-22)The view on the significance of the presence of glycans in glycoconjugates is undergoing a paradigmatic change. Initially mostly considered to be rather inert and passive, the concept of the sugar code identifies glycans ... -
Merging carbohydrate chemistry with lectin histochemistry to study inhibition of lectin binding by glycoclusters in the natural tissue context
(Springer, 2015-11-09)Recognition of glycans by lectins leads to cell adhesion and growth regulation. The specificity and selectivity of this process are determined by carbohydrate structure (sequence and shape) and topology of its presentation. ... -
Multiple myeloma: The role of glycosylation in disease development and progression.
(2017-09-09)Multiple myeloma (MM) is a clonal plasma cell disorder that is characterized by skeletal destruction, renal failure, anaemia and hypercalcaemia. MM cells are intimately associated with the bone marrow microenvironment, ... -
N- and O- linked Glycosylation, Developing Mass Spectrometric Strategies for the Characterisation of Glyco-epitopes
(2012-12-10)The overall goal of this thesis was to adopt a liquid chromatography-mass spectrometry (LC-MS) platform for glycomic analysis of glycoproteins. In particular we were interested in characterizing complex glycomic samples ... -
Neuronal glycosylation differentials in normal, injured and chondroitinase-treated environments
(2012)Glycosylation is found ubiquitously throughout the central nervous system (CNS). Chondroitin sulphate proteoglycans (CSPGs) are a group of molecules heavily substituted with glycosaminoglycans (GAGs) and are found in the ... -
The role of glycosylation in intervertebral disc degeneration: Towards glyco-functionalised therapeutics
(NUI Galway, 2021-09-24)Low back pain (LBP) is a common health issue that imposes a significant socio-economic burden on society. Primarily, intervertebral disc degeneration is one of the major contributing causes of LBP. It is initiated by altered ... -
The role of glycosylation in regulatory T-cell biology
(2016-12-14)Regulatory T-cells (T-reg) play a pivotal role in maintaining immune homeostasis and tolerance. There has been a significant amount of research devoted to understanding their physiological mechanisms of action and the ... -
Surface chemistry and linker effects on lectin-carbohydrate recognition for glycan microarrays
(2012)Glycan microarrays are an increasingly utilised tool for analysis of protein-carbohydrate interactions and a variety of glycan-containing molecules and slide chemistries have been used to array carbohydrates on microarray ... -
Targeting the glycome of major milk proteins
(2016-10-20)The objectives of this thesis were to 1) explore the glycosylation of major bovine milk proteins, 2) investigate the effect of lactational stage on glycan structures and 3) examine how these documented changes may influence ... -
Teaming up synthetic chemistry and histochemistry for activity screening in galectin-directed inhibitor design
(Springer, 2016-06-24)A hallmark of endogenous lectins is their ability to select a few distinct glycoconjugates as counterreceptors for functional pairing from the natural abundance of cellular glycoproteins and glycolipids. As a consequence, ...