Browsing by Subject "DNA"
Now showing items 1-20 of 40
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A cenp-s/x complex assembles at the centromere in s and g2 phases of the human cell cycle
(The Royal Society, 2014-02-12)The functional identity of centromeres arises from a set of specific nucleoprotein particle subunits of the centromeric chromatin fibre. These include CENP-A and histone H3 nucleosomes and a novel nucleosome-like complex ... -
Assessing the microbial oxidative stress mechanism of ozone treatment through the responses of escherichia coli mutants
(Wiley-Blackwell, 2011-05-13)Aims: To investigate the effect of the oxidative stress of ozone on the microbial inactivation, cell membrane integrity and permeability and morphology changes of Escherichia coli. Methods and Results: Escherichia coli BW ... -
Association of foreign dna with porcine spermatozoa
(Informa UK Limited, 1991-01-01)The aim of this project was to establish if DNA molecules bind to procine spermatozoa after incubation in vitro. In an initial experiment, the DNA was mixed with porcine spermatozoa to test for the presence of DNases. ... -
Birth, evolution, and transmission of satellite-free mammalian centromeric domains
(Cold Spring Harbor Laboratory, 2018-04-30)Mammalian centromeres are associated with highly repetitive DNA (satellite DNA), which has so far hindered molecular analysis of this chromatin domain. Centromeres are epigenetically specified, and binding of the CENPA ... -
Characterization of two autoreplicative regions of the inchi2 plasmid r478: rephi2a and rephi1a(r478)
(Microbiology Society, 2001-06-01)Plasmids of the incompatibility groups HI and HII (IncH plasmids) generally confer multiple antibiotic resistances upon their host pathogenic strain. IncHI group plasmids are distinguished by their property of optimal ... -
Cloning, overexpression in escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from talaromyces emersonii
(American Society for Microbiology, 2012-03-09)The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces etnersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased ... -
Construction of synthetic nucleoli and what it tells us about propagation of sub-nuclear domains through cell division
(Informa UK Limited, 2014-08-18)The cell nucleus is functionally compartmentalized into numerous membraneless and dynamic, yet defined, bodies. The cell cycle inheritance of these nuclear bodies (NBs) is poorly understood at the molecular level. In higher ... -
Construction of synthetic nucleoli in human cells reveals how a major functional nuclear domain is formed and propagated through cell division
(Cold Spring Harbor Laboratory, 2014-01-21)Human cell nuclei are functionally organized into structurally stable yet dynamic bodies whose cell cycle inheritance is poorly understood. Here, we investigate the biogenesis and propagation of nucleoli, sites of ribosome ... -
Determination of the number of RAD51 molecules in different human cell lines
(Taylor & Francis, 2019-11-15)Knowledge about precise numbers of specific molecules is necessary for understanding and verification of biological pathways. The RAD51 protein is central in the repair of DNA double-strand breaks (DSBs) by homologous ... -
Development of a rapid recombinase polymerase amplification assay for the detection of Streptococcus pneumoniae in whole blood
(BioMed Central, 2015-10-29)Background: Streptococcus pneumoniae is an important cause of microbial disease in humans. The introduction of multivalent vaccines has coincided with a dramatic decrease in the number of pneumococcal-related deaths. In ... -
Development of a rapid recombinase polymerase amplification assay for the detection of streptococcus pneumoniae in whole blood
(Springer Nature, 2015-10-29)Background: Streptococcus pneumoniae is an important cause of microbial disease in humans. The introduction of multivalent vaccines has coincided with a dramatic decrease in the number of pneumococcal-related deaths. In ... -
DNA hybridisation detection on chemically modified electrodes with bioelectrocatalytic amplification of signal
(2011-12-22)Electrochemical detection of DNA hybridization is a viable alternative to optical and radio-labelled techniques. The benefits of using biosensors based on electrochemical systems are: high sensitivity, possible development ... -
Draft genome sequence of pigeonpea (cajanus cajan), an orphan legume crop of resource-poor farmers
(Springer Nature, 2011-11-06)Pigeonpea is an important legume food crop grown primarily by smallholder farmers in many semi-arid tropical regions of the world. We used the Illumina next-generation sequencing platform to generate 237.2 Gb of sequence, ... -
Effects of electropermeabilization on the association of foreign dna with pig sperm
(Informa UK Limited, 1992-01-01)This study was conducted to evaluate the effects of an electric pulse (electroporation/electropermeabilization) on the binding of foreign DNA molecules to porcine spermatozoa. We previously examined various parameters ... -
Electrocatalytic oxidation of alcohols, tripropylamine, and dna with ligand-free gold nanoclusters on nitrided carbon
(Wiley-Blackwell, 2016-07-20)Electrocatalytic properties of ligand-free gold nanoclusters (AuNCs, <2nm) grown on nitrided carbon supports (denoted as AuNCs@N-C) were evaluated for the oxidation of representative organic molecules including ... -
Electrochemical activation of the natural catalytic cycle of cytochrome p450s in human liver microsomes
(Wiley-Blackwell, 2012-10-16)The natural catalytic cycle of cytochrome (cyt) P450 enzymes in human liver microsome (HLM) films was activated electrochemically via the electron transfer sequence electrode -> cyt P450 reductase (CPR)-> cyt ... -
Gene-eluting stents: non-viral, liposome-based gene delivery of enos to the blood vessel wall in vivo results in enhanced endothelialization but does not reduce restenosis in a hypercholesterolemic model
(Springer Nature, 2011-06-30)Although successful, drug-eluting stents require significant periods of dual anti-platelet therapy with a persistent risk of late stent thrombosis due to inhibition of re-endothelialization. Endothelial regeneration is ... -
Hiv type 1 mutational patterns in hiv type 1 subtype c-infected long-term survivors in karonga district malawi: further analysis and correction
(Mary Ann Liebert Inc, 2012-03-01)Here we present new sequence data from HIV-1 subtype C-infected long-term survivors (LTS) from Karonga District, Malawi. Gag and env sequence data were produced from nine individuals each of whom has been HIV-1 positive ... -
Identification and characterization of fusarium mangiferae as pathogen of mango malformation in india
(FapUNIFESP (SciELO), 2016-01-01)Fusarium mangiferae (=F. subglutinans) isolates collect from malformed samples from major mango-growing area of North India. Molecular identification and characterization of eleven most virulent isolates of F. mangiferae, ... -
Impacts of DNA damage on the pericentriolar material and centriole duplication
(2015-12-03)The centrosome consists of two barrel-shaped centrioles embedded in a proteinaceous pericentriolar material (PCM), a highly organised scaffold that serves as a platform for proteins that regulate spindle assembly, protein ...