Browsing by Author "Barry, Thomas"
Now showing items 1-20 of 28
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A novel multiplex real-time pcr for the identification of mycobacteria associated with zoonotic tuberculosis
Reddington, Kate; O'Grady, Justin; Dorai-Raj, Siobhan; Niemann, Stefan; van Soolingen, Dick; Barry, Thomas (Public Library of Science (PLoS), 2011-08-09)Background: Tuberculosis (TB) is the leading cause of death worldwide from a single infectious agent. An ability to detect the Mycobacterium tuberculosis complex (MTC) in clinical material while simultaneously differentiating ... -
A rapid culture independent methodology to quantitatively detect and identify common human bacterial pathogens associated with contaminated high purity water
Minogue, Elizabeth; Tuite, Nina L; Smith, Cindy J; Reddington, Kate; Barry, Thomas (Springer Nature, 2015-01-01)Background: Water and High Purity Water (HPW) distribution systems can be contaminated with human pathogenic microorganisms. This biocontamination may pose a risk to human health as HPW is commonly used in the industrial, ... -
Comparative genome analysis identifies novel nucleic acid diagnostic targets for use in the specific detection of Haemophilus influenzae
Coughlan, Helena; Reddington, Kate; Tuite, Nina; Wee Boo, Teck; Cormican, Martin; Barrett, Louise; Smith, Terry; Clancy, Eoin; Barry, Thomas (Elsevier, 2015-06-23)Haemophilus influenzae is recognised as an important human pathogen associated with invasive infections, including bloodstream infection and meningitis. Currently used molecular-based diagnostic assays lack specificity in ... -
Comparison of established diagnostic methodologies and a novel bacterial smpB real-time PCR assay for the specific detection of haemophilus influenzae associated with respiratory tract infections
Reddington, Kate; Schwenk, Stefan; Tuite, Nina; Platt, Gareth; Davar, Danesh; Coughlan, Helena; Personne, Yoann; Enne, Virve I.; Zumla, Alimuddin; Barry, Thomas (American Society for Microbiology, 2015-06-23)Haemophilus influenzae is a significant causative agent of respiratory tract infections (RTI) worldwide. The development of a rapid H. influenzae diagnostic assay that would allow for the implementation of infection control ... -
Comparison of established diagnostic methodologies and a novel bacterialsmpbreal-time pcr assay for specific detection of haemophilus influenzae isolates associated with respiratory tract infections
Reddington, Kate; Schwenk, Stefan; Tuite, Nina; Platt, Gareth; Davar, Danesh; Coughlan, Helena; Personne, Yoann; Gant, Vanya; Enne, Virve I.; Zumla, Alimuddin; Barry, Thomas (American Society for Microbiology, 2015-06-24)Haemophilus influenzae is a significant causative agent of respiratory tract infections (RTI) worldwide. The development of a rapid H. influenzae diagnostic assay that would allow for the implementation of infection control ... -
Cross platform standardisation of an experimental pipeline for use in the identification of dysregulated human circulating MiRNAs
Kelly, Helena; Downing, Tim; Tuite, Nina; Smith, Terry; Kerin, Michael; Dwyer, Róisín M.; Clancy, Eoin; Reddington, Kate; Barry, Thomas (Public Library of Science, 2015-09-10)Micro RNAs (miRNAs) are a class of highly conserved small non-coding RNAs that play an important part in the post-transcriptional regulation of gene expression. A substantial number of miRNAs have been proposed as biomarkers ... -
Cross platform standardisation of an experimental pipeline for use in the identification of dysregulated human circulating mirnas
Kelly, Helena; Downing, Tim; Tuite, Nina L.; Smith, Terry J.; Kerin, Michael J.; Dwyer, Róisín M.; Clancy, Eoin; Barry, Thomas; Reddington, Kate (Public Library of Science (PLoS), 2015-09-10)Introduction Micro RNAs (miRNAs) are a class of highly conserved small non-coding RNAs that play an important part in the post-transcriptional regulation of gene expression. A substantial number of miRNAs have been proposed ... -
Design, development and validation of a series of multiplex real-time PCR diagnostics assays for the rapid and accurate detection and differentiation of the Mycobacterium tuberculosis complex
Reddington, Kate (2012-03-01)Tuberculosis (TB) remains a major health concern both in developed and developing countries due to the high rates of morbidity and mortality associated with disease. In humans, TB is caused by members of the Mycobacterium ... -
Detection of nasba amplified bacterial tmrna molecules on slicsel designed microarray probes
Scheler, Ott; Kaplinski, Lauris; Glynn, Barry; Palta, Priit; Parkel, Sven; Toome, Kadri; Maher, Majella; Barry, Thomas; Remm, Maido; Kurg, Ants (Springer Nature, 2011-01-01)Background: We present a comprehensive technological solution for bacterial diagnostics using tmRNA as a marker molecule. A robust probe design algorithm for microbial detection microarray is implemented. The probes were ... -
Development and validation of a rapid real-time pcr based method for the specific detection of salmonella on fresh meat
McGuinness, Sheila; McCabe, Evonne; O’Regan, Edel; Dolan, Anthony; Duffy, Geraldine; Burgess, Catherine; Fanning, Seamus; Barry, Thomas; O’Grady, Justin (Elsevier BV, 2009-11-01) -
Development of a rapid recombinase polymerase amplification assay for the detection of Streptococcus pneumoniae in whole blood
Clancy, Eoin; Higgins, Owen; Boo, Teck Wee; Cormican, Martin; Barry, Thomas; Smith, Terry J. (BioMed Central, 2015-10-29)Background: Streptococcus pneumoniae is an important cause of microbial disease in humans. The introduction of multivalent vaccines has coincided with a dramatic decrease in the number of pneumococcal-related deaths. In ... -
Development of a rapid recombinase polymerase amplification assay for the detection of streptococcus pneumoniae in whole blood
Clancy, Eoin; Higgins, Owen; Forrest, Matthew S.; Boo, Teck Wee; Cormican, Martin; Barry, Thomas; Piepenburg, Olaf; Smith, Terry J. (Springer Nature, 2015-10-29)Background: Streptococcus pneumoniae is an important cause of microbial disease in humans. The introduction of multivalent vaccines has coincided with a dramatic decrease in the number of pneumococcal-related deaths. In ... -
Development of a real-time multiplex pcr assay for the detection of multiple salmonella serotypes in chicken samples
O'Regan, Edel; McCabe, Evonne; Burgess, Catherine; McGuinness, Sheila; Barry, Thomas; Duffy, Geraldine; Whyte, Paul; Fanning, Séamus (Springer Nature, 2008-01-01)Background: A real-time multiplex PCR assay was developed for the detection of multiple Salmonella serotypes in chicken samples. Poultry-associated serotypes detected in the assay include Enteritidis, Gallinarum, Typhimurium, ... -
Development of an on-disc isothermal in vitro amplification and detection of bacterial rna
Brennan, Des; Coughlan, Helena; Clancy, Eoin; Dimov, Nikolay; Barry, Thomas; Kinahan, David; Ducrée, Jens; Smith, Terry J.; Galvin, Paul (Elsevier BV, 2017-02-01) -
Development of novel culture independent nucleic acid based diagnostics technologies for the detection and identification of microbial contaminants in building water distribution and premise plumbing systems
Minogue, Elizabeth (2015-02-22)Water is a vital component of the industrial, pharmaceutical and healthcare sectors. In industrial and pharmaceutical sectors water is a commonly used raw material, solvent and ingredient. While in healthcare facilities ... -
Development of novel nucleic acid diagnostics technologies for the detection of predominant microorganisms associated with bacterial meningitis
Coughlan, Helena (2017-01-13)With an estimated 1.2 million cases annually worldwide, bacterial meningitis is a medical emergency and primary prevention as well as accurate diagnosis and treatment of this often fatal disease is paramount. The ... -
Evaluation of a novel real-time PCR test based on the ssrA gene for the identification of group B streptococci in vaginal swabs.
Wernecke, Martina; Mullen, Ciara; Sharma, Vimla; Morrison, John; Barry, Thomas; Maher, Majella; Smith, Terry (2009)Despite the implementation of prevention guidelines, early-onset group B streptococci (GBS) disease remains a cause of neonatal morbidity and mortality worldwide. Strategies to identify women who are at risk of transmitting ... -
Evaluation of a novel real-time pcr test based on the ssragene for the identification of group b streptococci in vaginal swabs
Wernecke, Martina; Mullen, Ciara; Sharma, Vimla; Morrison, John; Barry, Thomas; Maher, Majella; Smith, Terry (Springer Nature, 2009-09-04)Background: Despite the implementation of prevention guidelines, early-onset group B streptococci (GBS) disease remains a cause of neonatal morbidity and mortality worldwide. Strategies to identify women who are at risk ... -
Label-free, multiplexed detection of bacterial tmrna using silicon photonic microring resonators
Scheler, Ott; Kindt, Jared T.; Qavi, Abraham J.; Kaplinski, Lauris; Glynn, Barry; Barry, Thomas; Kurg, Ants; Bailey, Ryan C. (Elsevier BV, 2012-06-01) -
Novel Multiplex Real-Time PCR Diagnostic Assay for Identification and Differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis Complex Strains
Reddington, Kate; O'Grady, Justin; Dorai-Raj, Siobhan; Maher, Majella; van Soolingen, Dick; Barry, Thomas (Journal of Clinical Microbiology, 2011-02)Tuberculosis (TB) in humans is caused by members of the Mycobacterium tuberculosis complex (MTC). Rapid detection of the MTC is necessary for the timely initiation of antibiotic treatment, while differentiation between ...