dc.contributor.author | Broderick, Ronan | |
dc.contributor.author | Ramadurai, Sivaramakrishnan | |
dc.contributor.author | Tóth, Katalin | |
dc.contributor.author | Togashi, Denisio M. | |
dc.contributor.author | Ryder, Alan G. | |
dc.contributor.author | Langowski, Jörg | |
dc.contributor.author | Nasheuer, Heinz-Peter | |
dc.date.accessioned | 2021-01-07T09:45:29Z | |
dc.date.available | 2021-01-07T09:45:29Z | |
dc.date.issued | 2012-04-19 | |
dc.identifier.citation | Broderick R, Ramadurai S, Tóth K, Togashi DM, Ryder AG, Langowski J, et al. (2012) Cell Cycle-Dependent Mobility of Cdc45 Determined in vivo by Fluorescence Correlation Spectroscopy. PLoS ONE 7(4): e35537. https://doi.org/10.1371/journal.pone.0035537 | en_IE |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/10379/16422 | |
dc.description.abstract | Eukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS) in vivo in asynchronous cells and in cells synchronized at the G1/S transition and during S phase. Our data show that eGFP-Cdc45 mobility is faster in G1/S transition compared to S phase suggesting that Cdc45 is part of larger protein complex formed in S phase. Furthermore, the size of complexes containing Cdc45 was estimated in asynchronous, G1/S and S phase-synchronized cells using gel filtration chromatography; these findings complemented the in vivo FCS data. Analysis of the mobility of eGFP-Cdc45 and the size of complexes containing Cdc45 and eGFP-Cdc45 after UVC-mediated DNA damage revealed no significant changes in diffusion rates and complex sizes using FCS and gel filtration chromatography analyses. This suggests that after UV-damage, Cdc45 is still present in a large multi-protein complex and that its mobility within living cells is consistently similar following UVC-mediated DNA damage. | en_IE |
dc.format | application/pdf | en_IE |
dc.language.iso | en | en_IE |
dc.publisher | Public Library of Science | en_IE |
dc.relation.ispartof | Plos One | en |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | EUKARYOTIC DNA-REPLICATION | en_IE |
dc.subject | PROTEIN-A | en_IE |
dc.subject | POLYMERASE-ALPHA | en_IE |
dc.subject | MCM2-7 HELICASE | en_IE |
dc.subject | S-PHASE | en_IE |
dc.subject | COMPLEX | en_IE |
dc.subject | INITIATION | en_IE |
dc.subject | DYNAMICS | en_IE |
dc.subject | CHROMATIN | en_IE |
dc.subject | GINS | en_IE |
dc.title | Cell cycle-dependent mobility of Cdc45 determined in vivo by fluorescence correlation spectroscopy | en_IE |
dc.type | Article | en_IE |
dc.date.updated | 2021-01-05T21:40:06Z | |
dc.identifier.doi | 10.1371/journal.pone.0035537 | |
dc.local.publishedsource | https://doi.org/10.1371/journal.pone.0035537 | en_IE |
dc.description.peer-reviewed | peer-reviewed | |
dc.internal.rssid | 2194649 | |
dc.local.contact | Heinz-Peter Nasheuer, 2fn, Rm Bms 2010, Biochemistry S, Bioresearch Building, Nui Galway, Galway. 2430 Email: heinz.nasheuer@nuigalway.ie | |
dc.local.copyrightchecked | Yes | |
dc.local.version | PUBLISHED | |
nui.item.downloads | 90 | |