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Generation of a panel of high affinity antibodies and development of a biosensor-based immunoassay for the detection of okadaic acid in shellfish

dc.contributor.authorLe Berre, Marie
dc.contributor.authorKilcoyne, Michelle
dc.contributor.authorKane, Marian
dc.date.accessioned2020-03-16T09:46:17Z
dc.date.available2020-03-16T09:46:17Z
dc.date.issued2015-07-10
dc.identifier.citationLe Berre, Marie, Kilcoyne, Michelle, & Kane, Marian. (2015). Generation of a panel of high affinity antibodies and development of a biosensor-based immunoassay for the detection of okadaic acid in shellfish. Toxicon, 103, 169-175. doi:https://doi.org/10.1016/j.toxicon.2015.06.030en_IE
dc.identifier.issn879-3150
dc.identifier.urihttp://hdl.handle.net/10379/15850
dc.description.abstractOkadaic acid (OA) and its derivatives, DTX-1 and DTX-2, are marine biotoxins associated with diarrhetic shellfish poisoning. Routine monitoring of these toxins relies on the mouse bioassay. However, due to the technical unreliability and animal usage of this bioassay, there is always a need for convenient and reliable alternative assay methods. A panel of monoclonal antibodies against OA was generated and the most suitable was selected for biosensor-based assay development using surface plasmon resonance. The cross reactivity of the selected antibody with DTX-1 was found to be 73%, confirming the antibody suitability for both OA and DTX detection. The OA and derivative assay was designed as an inhibition assay covering the concentrations 1-75 ng/ml, with a sensitivity of 22.4 ng/ml. The assay was highly reproducible and preliminary validation showed no matrix interference from mussel extracts and good recovery of added standard in mussel extracts, with %CV ofen_IE
dc.description.sponsorshipThis work was supported by grants from the Irish Higher Education Authority’s Programme for Research in Third Level Institutions (PRTLI), as part of the Marine Science Research Programme in the Ryan Institute, NUI Galway, Ireland and from Advanced Technologies Research Programme of Enterprise Ireland. The authors would like to thank Dr. Philipp Hess from the Marine Institute, Galway, Ireland, for the extraction protocol and contaminated mussels.en_IE
dc.language.isoenen_IE
dc.publisherElsevieren_IE
dc.relation.ispartofToxiconen
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectBiosensoren_IE
dc.subjectOkadaic aciden_IE
dc.subjectDSPen_IE
dc.subjectMonoclonal antibodyen_IE
dc.subjectShellfishen_IE
dc.subjectImmunoassayen_IE
dc.subjectPHOSPHATASE INHIBITION ASSAYen_IE
dc.subjectMONOCLONAL-ANTIBODYen_IE
dc.subjectQUANTITATIVE-DETERMINATIONen_IE
dc.subjectLABORATORY VALIDATIONen_IE
dc.subjectTOXINSen_IE
dc.subjectIMMUNOSENSORen_IE
dc.subjectTOXICITYen_IE
dc.subjectMUSSELSen_IE
dc.titleGeneration of a panel of high affinity antibodies and development of a biosensor-based immunoassay for the detection of okadaic acid in shellfishen_IE
dc.typeArticleen_IE
dc.date.updated2020-03-16T09:03:31Z
dc.identifier.doi10.1016/j.toxicon.2015.06.030
dc.local.publishedsourcehttps://doi.org/10.1016/j.toxicon.2015.06.030en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderEnterprise Irelanden_IE
dc.contributor.funderProgramme for Research in Third Level Institutions (PRTLI)en_IE
dc.internal.rssid9451550
dc.local.contactMichelle Kilcoyne, Glycosciences, School Of Natural Sciences, Nui Galway. 5885 Email: michelle.kilcoyne@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Ireland