dc.contributor.author | Collins, Caitríona M. | |
dc.contributor.author | Malacrida, Beatrice | |
dc.contributor.author | Burke, Colin | |
dc.contributor.author | Kiely, Patrick A. | |
dc.contributor.author | Dunleavy, Elaine M. | |
dc.date.accessioned | 2018-09-20T16:03:46Z | |
dc.date.available | 2018-09-20T16:03:46Z | |
dc.date.issued | 2018-07-13 | |
dc.identifier.citation | Collins, Caitríona M. Malacrida, Beatrice; Burke, Colin; Kiely, Patrick A.; Dunleavy, Elaine M. (2018). Atp synthase f1 subunits recruited to centromeres by cenp-a are required for male meiosis. Nature Communications 9 , | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | http://hdl.handle.net/10379/10854 | |
dc.description.abstract | The histone H3 variant CENP-A epigenetically defines the centromere and is critical for chromosome segregation. Here we report an interaction between CENP-A and subunits of the mitochondrial ATP synthase complex in the germline of male Drosophila. Furthermore, we report that knockdown of CENP-A, as well as subunits ATPsyn-alpha, -beta like (a testis-specific paralogue of ATPsyn-beta) and -gamma disrupts sister centromere cohesion in meiotic prophase I. We find that this disruption is likely independent of reduced ATP levels. We identify that ATPsyn-alpha and -beta like localise to meiotic centromeres and that this localisation is dependent on the presence of CENP-A. We show that ATPsyn-alpha directly interacts with the N-terminus of CENP-A in vitro and that truncation of its N terminus perturbs sister centromere cohesion in prophase I. We propose that the CENP-A N-terminus recruits ATPsyn-alpha and -beta like to centromeres to promote sister centromere cohesion in a nuclear function that is independent of oxidative phosphorylation. | |
dc.publisher | Springer Nature | |
dc.relation.ispartof | Nature Communications | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | sister-chromatid cohesion | |
dc.subject | drosophila-melanogaster | |
dc.subject | shugoshin mei-s332 | |
dc.subject | protein | |
dc.subject | localization | |
dc.subject | spermatogenesis | |
dc.subject | histone | |
dc.title | Atp synthase f1 subunits recruited to centromeres by cenp-a are required for male meiosis | |
dc.type | Article | |
dc.identifier.doi | 10.1038/s41467-018-05093-9 | |
dc.local.publishedsource | https://www.nature.com/articles/s41467-018-05093-9.pdf | |
nui.item.downloads | 0 | |